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通过硅烷反应将信号蛋白 3A 壳聚糖膜固定化到微弧氧化钛植入表面，以提高 MG63 成骨分化

 

Authors Fang K, Song W, Wang L, Jia S, Wei H, Ren S, Xu X, Song Y

Published Date October 2014 Volume 2014:9(1) Pages 4649—4657

DOI http://dx.doi.org/10.2147/IJN.S68895

Received 5 June 2014, Accepted 19 July 2014, Published 3 October 2014

Abstract: Improving osseointegration of extensively used titanium (Ti) implants still remains a main theme in implantology. Recently, grafting biomolecules onto a Ti surface has attracted more attention due to their direct participation in the osseointegration process around the implant. Semaphorin 3A (Sema3A) is a new proven osteoprotection molecule and is considered to be a promising therapeutic agent in bone diseases, but how to immobilize the protein onto a Ti surface to acquire a long-term effect is poorly defined. In our study, we tried to use chitosan to wrap Sema3A (CS/Sema) and connect to the microarc oxidized Ti surface via silane glutaraldehyde coupling. The microarc oxidization could formulate porous topography on a Ti surface, and the covalently bonded coating was homogeneously covered on the ridges between the pores without significant influence on the original topography. A burst release of Sema3A was observed in the first few days in phosphate-buffered saline and could be maintained for >2 weeks. Coating in phosphate-buffered saline containing lysozyme was similar, but the release rate was much more rapid. The coating did not significantly affect cellular adhesion, viability, or cytoskeleton arrangement, but the osteogenic-related gene expression was dramatically increased and calcium deposition was also abundantly detected. In conclusion, covalent bonding of CS/Sema could strongly improve osteogenic differentiation of osteoblasts and might be applied for Ti implant surface biofunctionalization.
Keywords: titanium, semaphorin 3A, silane reaction, microarc oxidation, osteogenic differentiation







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