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MiR-200a-3p 通过调节 PCDH9 促进卵巢癌细胞的恶性行为
Authors Shi C, Yang Y, Zhang L, Yu J, Qin S, Xu H, Gao Y
Received 21 June 2019
Accepted for publication 13 August 2019
Published 8 October 2019 Volume 2019:12 Pages 8329—8338
DOI https://doi.org/10.2147/OTT.S220339
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Shashank Kaushik (PT)
Peer reviewer comments 2
Editor who approved publication: Dr Sanjeev Srivastava
Background: Increasing evidence has revealed that the aberrant expression of microRNAs (miRNAs) plays vital roles in the development and progression of ovarian cancer. MiR-200a-3p was found to act as an oncogene in a variety of cancers, however, the expression and function of miR-200a-3p in ovarian cancer has not been characterized.
Materials and methods: The expression of miR-200a-3p in ovarian cancer tissues and cell lines was detected by the RT-qPCR. The influence of miR-200a-3p on the growth of ovarian cancer cells was determined with the Cell Counting Kit-8 assay, colony formation and cell invasion assay. The binding of miR-200a-3p with the 3ʹ-untranslated region (UTR) of PDCH9 was detected by luciferase reporter assay. The expression of PCDH9 was investigated by RT-qPCR and Western blot analysis.
Results: miR-200a-3p was up-regulated in ovarian cancer tissues and cell lines. Highly expressed miR-200a-3p was significantly associated with the tumor size, tumor metastasis and TNM stage. Overexpression of miR-200a-3p markedly promoted the proliferation, colony formation and invasion of ovarian cancer cells. Functional study uncovered that miR-200a-3p bound the 3ʹ-untranslated region (UTR) of PCDH9 and decreased the expression of PCDH9 in ovarian cancer cells. The expression of miR-200a-3p in ovarian cancer tissues was significantly negatively correlated with that of PCDH9. Restored PCDH9 inhibited the promoting effect of miR-200a-3p on the proliferation of ovarian cancer cells.
Conclusion: Our results suggested the potential oncogenic function of miR-200a-3p via modulating PCDH9 in ovarian cancer.
Keywords: miR-200a-3p, ovarian cancer, PCDH9