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通过流式细胞术所检测到的壶腹周围或胰腺癌中的门静脉循环肿瘤细胞进行术后转移预测
Authors Tao L, Su L, Yuan C, Ma Z, Zhang L, Bo S, Niu Y, Lu S, Xiu D
Received 28 March 2019
Accepted for publication 20 June 2019
Published 6 August 2019 Volume 2019:11 Pages 7405—7425
DOI https://doi.org/10.2147/CMAR.S210332
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 2
Editor who approved publication: Dr Antonella D'Anneo
Purpose: The aim of this study was to evaluate the value of flow cytometry (FCM) detection of portal vein circulating tumor cells (CTCs) in predicting postoperative metastasis.
Methods: Samples of portal venous blood and peripheral blood were collected from 39 patients during surgery, and CTCs were detected by FCM, with confirmation by laser confocal microscopy and single-cell sequencing.
Results: Among all patients, a portal EpCAM+CD45- percentage ≥24.5×10−4 (P =0.06), peripheral EpCAM+CD45- count ≥97/5 mL (P =0.034), peripheral EpCAM+CD45- percentage ≥4.4×10−4(P =0.042), and CA242≥3.5 U/mL (P =0.027) were significant predictors of metastasis. Further analysis showed that the portal EpCAM+CD45- ratio ≥24.5×10−4 is a predictor of metastasis (P =0.025) in pancreatic cancer after curative resection.
Conclusion: CTCs detected by FCM in portal venous blood are of significant value for the prediction of postoperative metastasis in pancreatic or periampullary tumors.
Keywords: circulating tumor cells, flow cytometry, portal vein, pancreatic cancer, metastases