已发表论文

TGF-β1 介导 lncRNA GAPLINC 表达促进非小细胞肺癌的迁移和侵袭

 

Authors Zhao J, Wang C, Liu S, Su X, Ouyang A

Received 28 February 2019

Accepted for publication 12 June 2019

Published 2 August 2019 Volume 2019:12 Pages 6175—6180

DOI https://doi.org/10.2147/OTT.S207079

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Ms Aruna Narula

Peer reviewer comments 2

Editor who approved publication: Dr Carlos E Vigil

Purpose: The present study aims to investigate the involvement of lncRNA GAPLINC in non-small lung cancer (NSCLC).
Patients and methods: The study included 70 patients with NSCLC (39 males and 31 females, 33 to 68 years, 49.3 ± 6.4 years). RT-qPCR, transient cell transfections, measurement of in vitro cell migration and invasion abilities and western blot were carrying out during the research.
Results: We showed that GAPLINC was up-regulated in NSCLC tissues and positively correlated with TGF-β1. In vitro cell experiment showed that over-expression of TGF-β1 significantly up-regulated the expression of GAPLINC, while over-expression of GAPLINC failed to affect TGF-β1. Follow-up study showed that high GAPLINC level in NSCLC tissue was closely correlated with poor survival rate of NSCLC patients. Over-expressions of TGF-β1 and GAPLINC resulted to accelerated migration and invasion of NSCLC cells. In addition, the silencing of GAPLINC siRNA attenuated the effect of TGF-β1 treatment.
Conclusion: TGF-β1 may mediate lncRNA GAPLINC expression to promote NSCLC cell invasion and migration.
Keywords: non-small cell lung cancer, lncRNA GAPLINC, TGF-β1, prognosis




Figure 4 TGF-β1 promoted GAPLINC expression to accelerate non-small cell lung cancer...