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已发表论文
miR203 的下调导致了PIK3CA 过表达并预测了胃癌患者较差的预后
Authors Liang M, Shi B, Liu J, He L, Yi G, Zhou L, Yu G, Zhou X
Published Date July 2015 Volume 2015:9 Pages 3607—3616
DOI http://dx.doi.org/10.2147/DDDT.S85525
Received 26 March 2015, Accepted 8 May 2015, Published 16 July 2015
Background: Despite advances in clinical therapies and technologies, the prognosis
for patients with gastric cancer is still poor. The aim of this study is to
investigate new predictive markers for prognosis of gastric cancer.
Methods: In this study, we evaluated the expression pattern of PIK3CA in 107 gastric cancer specimens and their adjacent nontumorous tissues. PIK3CA siRNA was synthesized and transfected into gastric cancer cell lines. Colony formation and MTT assays were employed to analyze the cell proliferation. PIK3CA expression was examined by using immunohistochemical analysis and Western blot assay. Transwell invasion assay was used to detect the invasion capability of the cells. Luciferase activity was examined by using 3'-untranslated region luciferase reporter assays.
Results: We observed that PIK3CA was significantly upregulated in gastric cancer tissues. High expression level of PIK3CA was detectable in 48 (44.86%) of the gastric cancer specimens, and correlated with poor prognosis. In addition, our study indicated that miR203 inhibits cell proliferation and invasion via directly targeting and suppressing the PIK3CA expression. MiR203 expression is downregulated in gastric cancer tissues. Moreover, low expression level of miR203 predicted poor prognosis of gastric patients and induced overexpression of PIK3CA . Our further study also reported that overexpression of miR203 inhibited phosphorylation of AKT, while cotransfection of PIK3CA reversed the effect of miR203.
Conclusion: Our study suggested a miR203-PIK3CA -AKT signaling pathway in gastric cancer cells. This signaling pathway might play an important role in gastric cancer genesis and development.
Keywords: AKT, gastric cancer, miR203, PIK3CA , prognosis
Methods: In this study, we evaluated the expression pattern of PIK3CA in 107 gastric cancer specimens and their adjacent nontumorous tissues. PIK3CA siRNA was synthesized and transfected into gastric cancer cell lines. Colony formation and MTT assays were employed to analyze the cell proliferation. PIK3CA expression was examined by using immunohistochemical analysis and Western blot assay. Transwell invasion assay was used to detect the invasion capability of the cells. Luciferase activity was examined by using 3'-untranslated region luciferase reporter assays.
Results: We observed that PIK3CA was significantly upregulated in gastric cancer tissues. High expression level of PIK3CA was detectable in 48 (44.86%) of the gastric cancer specimens, and correlated with poor prognosis. In addition, our study indicated that miR203 inhibits cell proliferation and invasion via directly targeting and suppressing the PIK3CA expression. MiR203 expression is downregulated in gastric cancer tissues. Moreover, low expression level of miR203 predicted poor prognosis of gastric patients and induced overexpression of PIK3CA . Our further study also reported that overexpression of miR203 inhibited phosphorylation of AKT, while cotransfection of PIK3CA reversed the effect of miR203.
Conclusion: Our study suggested a miR203-PIK3CA -AKT signaling pathway in gastric cancer cells. This signaling pathway might play an important role in gastric cancer genesis and development.
Keywords: AKT, gastric cancer, miR203, PIK3CA , prognosis
