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Authors Cui LH, Xu HR, Yang W, Yu LJ
Received 28 June 2018
Accepted for publication 7 August 2018
Published 1 November 2018 Volume 2018:11 Pages 7715—7724
DOI https://doi.org/10.2147/OTT.S178597
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Tohru Yamada
Background: Investigating the roles of lncRNA prostate cancer-associated
transcript 6 (PCAT6) in modulating the growth and aggressiveness of
non-small-cell lung carcinoma (NSCLC) cell.
Method: The levels of PCAT6 in NSCLC tissues and cell lines were determined by
quantitative real-time PCR assay. MTT as well as colony formation assays were
applied to explore the effect of PCAT6 on the growth of NSCLC cell in vitro.
Wound healing and Transwell assays were utilized to analyze the impact of PCAT6
on the migration and invasion of NSCLC cell. Bioinformatics analysis and
luciferase reporter assay were used to prove that miR-330-5p was the target of
PCAT6. Colony formation, wound healing, and Transwell invasion assays were
applied to demonstrate that PCAT6 promoted NSCLC cell growth, migration, and
invasion through binding miR-330-5p. Finally, xenograft model was used to
explore the role of PCAT6 in the tumor growth of NSCLC cell in vivo.
Results: PCAT6 was highly overexpressed in NSCLC tissues and cells compared
with normal tissues and non-tumorigenic bronchial epithelial cell line,
BEAS-2B. Downregulation of PCAT6 markedly reduced the proliferation, migration,
and invasion of NSCLC cell. Moreover, down-expression of PCAT6 significantly
increased the level of miR-330-5p in NSCLC cell. Further functional experiments
indicated that down-expression of miR-330-5p reversed the inhibitory effect of
PCAT6 on NSCLC cell growth, migration, and invasion.
Conclusion: Our results reveal that lncRNA PCAT6 facilitates the
proliferation, migration, and invasion of NSCLC cell via competitively binding
to miR-330-5p.
Keywords: PCAT6, MiR-330-5p, NSCLC, growth, migration, invasion