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Authors Cai W, Lv W, Feng Y, Yang H, Zhang Y, Yang G, Duan Y, Wang J
Received 6 February 2018
Accepted for publication 5 June 2018
Published 24 October 2018 Volume 2018:13 Pages 6791—6807
DOI https://doi.org/10.2147/IJN.S164760
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Alexander Kharlamov
Peer reviewer comments 3
Editor who approved publication: Dr Lei Yang
Background: Nanobubbles (NBs) combined with ultrasound-targeted destruction
(UTD) have become promising potential carriers for drug or siRNA delivery. Due
to their nano-size, NBs could penetrate tumor blood vessels and accumulate in
intercellular spaces so that “sonoporation” induced by UTD would act directly
on the tumor cells to increase cell membrane permeability.
Methods: Based on the successful the fabrication of NBs, we synthesized NBs
carrying siRNA (NBs-siRNA) by using a biotin-streptavidin system. We then
utilized ultrasound irradiation (UI)-targeted NBs-siRNA to improve siRNA
transfection and achieve the inhibition of glioma growth.
Results: NBs as carriers combined with UI effectively enhanced siRNA
transfection and the effect of silencing targeted genes in vitro. Additionally,
a better therapeutic effect was shown in the NBs-siRNA with UI group in vivo
compared with that of microbubbles (MBs) with UI or NBs-siRNA without UI.
Conclusion: These results indicated that NBs combined with UTD might be an
ideal delivery vector for siRNA to achieve the noninvasive treatment of glioma.
Keywords: nanobubbles, ultrasound, siRNA, glioma