论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
Authors Dong B, Sun P, Ruan G, Huang W, Mao X, Kang Y, Pan D, Lin F
Received 10 July 2018
Accepted for publication 24 August 2018
Published 23 October 2018 Volume 2018:10 Pages 4839—4851
DOI https://doi.org/10.2147/CMAR.S179724
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 2
Editor who approved publication: Professor Nakshatri
Purpose: Currently, the associations between type-specific high-risk human
papillomavirus (HR-HPV) viral loads and cervical lesions are still
inconsistent. We aimed to assess the type-specific HR-HPV viral load as a risk
triage indicator for development of high-grade squamous intraepithelial lesion
or worse (≥HSIL).
Patients and
methods: A total of 19,446 women who
underwent primary screening for cervical cancer using Cervista® HR-HPV and cytology assays were enrolled. The viral loads of 1,396
HR-HPV-positive specimens confirmed by Cervista® assay were detected by BioPerfectus Multiplex Real-Time PCR assay.
The correlation between viral loads and cervical lesions was analyzed. The
optimal cutoffs of individual HR-HPV viral loads used to predict ≥HSIL were
determined from the receiver operating characteristic curve. A logistic
regression model was used to analyze the relationship between covariates and
the probability of ≥HSIL.
Results: The viral loads of HPV-16, -31, -33, -52, and -58 were positively
correlated with the severity of the cervical lesion, which was significantly
elevated in patients with ≥HSIL, whereas those of HPV-18, -45, -56, -59, and
other types were not. The optimal cutoffs of the log10-transformed viral loads for HPV-16, -31, -33, -52, and -58 in
identifying ≥HSIL were 4.26, 4.46, 4.48, 4.36, and 4.26 copies per 10,000
cells, respectively. Furthermore, multivariate analysis indicated that
type-specific viral loads of HPV-16, -31, -33, -52, and -58 exceeding the
cutoffs could be independent risk factors for the incidence of ≥HSIL.
Conclusion: The BioPerfectus Multiplex Real-Time PCR viral load assay provides
viable triage for ≥HSIL when using appropriate cutoff levels.
Keywords: high risk, human papillomavirus, viral load, high-grade squamous
intraepithelial lesion, BioPerfectus Multiplex Real-Time PCR