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Authors Sun C, Zhang X, Chen Y, Jia Q, Yang J, Shu Y
Received 23 November 2017
Accepted for publication 22 January 2018
Published 15 October 2018 Volume 2018:10 Pages 4581—4590
DOI https://doi.org/10.2147/CMAR.S157858
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Professor Kenan Onel
Background: A number of studies have indicated that expression of miRNA-365
(miR-365) is suppressed in various cancers, suggesting its cancer-suppressive
role. In the present investigation, we evaluated the regulation and character
of miR-365 in human esophageal squamous cell carcinoma (ESCC).
Patients and
methods: The tumor tissues and adjacent
nontumor tissue samples were collected from 30 patients having ESCC, and the
expression levels of miR-365 were studied by quantitative real-time polymerase
chain reaction (PCR). MTT and cell invasion by Matrigel assay were done to
study the effect of miR-365 on proliferation and metastasis of ESCC cells. An
in vivo tumor model was generated by inoculating ESCC cells subcutaneously into
BALB nude mice. A study of various biomarkers such as quantitative polymerase
chain reaction (qPCR), luciferase activity assay, and Western blot was done to
confirm the targets of miR-365.
Results: In tumor tissues, a significant downregulation of miR-365 was
observed versus the nontumor adjacent tissues and ESCC cells versus the
selected esophageal endothelial cells. It was observed that higher expression
levels of miR-365 inhibited the cell invasion, colony formation, growth in
esophageal cancer cell lines in vitro, and tumor development in vivo. The study
of biomarkers suggests involvement of phosphoserine aminotransferase 1 (PSAT1)
as a favorable target for miR-365, and its abnormal expression inverted the
miR-365-arbitrated suppression of invasion, viability, and
epithelial–mesenchymal transition in esophageal cancer cells. A negative
correlation existed with expression of miR-365 and PSAT1 in human esophageal
cancer tissue samples.
Conclusion: The study established that miR-365 exhibits tumor-suppressive
action via regulating the levels of PSAT1 and leads to invasion and
progressiveness of esophageal cancer.
Keywords: esophageal cancer, miR-365, PSAT1, tumor