论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
Authors Ding W, Ji T, Xiong W, Li T, Pu D, Liu R
Received 11 May 2018
Accepted for publication 2 July 2018
Published 15 October 2018 Volume 2018:12 Pages 3459—3469
DOI https://doi.org/10.2147/DDDT.S172525
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Qiongyu Guo
Purpose: In this study, we investigated the effect of Realgar on the
apoptosis of HPV16-positive cervical cells in vitro.
Methods: The effect of Realgar on the apoptosis of HPV16-positive cervical
cells was investigated by annexin V-fluorescein isothiocyanate/propidium iodide
staining and growth inhibition assays using HPV16-positive cervical cancer cell
line SiHa and HPV16-positive immortalized cervical epithelial cell line S12.
The expression of genes was measured by real-time PCR, and the expression of
corresponding proteins was detected by Western blotting. The adhesion and
invasion of cells were detected by adhesion assay and Transwell invasion assay,
respectively.
Results: The Realgar inhibited the proliferation and induced the apoptosis
of SiHa and S12 cells in a dose- and time-dependent manner. The Realgar
suppressed the expression of HPV16 E7 and caspase-3. The Realgar suppressed the
adhesion and invasion of both cells.
Conclusion: The Realgar induced apoptosis, inhibited the proliferation of
HPV16-positive cell lines through a HPV16 E7-dependent pathway, and inhibited
cell adhesion and invasion.
Keywords: Realgar, antitumor, human papillomavirus, E7