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已发表论文
miR-10a-5p 通过下调 Akt 途径抑制胆管癌细胞生长
Authors Gao L, Yang X, Zhang H, Yu M, Long J, Yang T
Received 1 August 2018
Accepted for publication 24 September 2018
Published 15 October 2018 Volume 2018:11 Pages 6981—6994
DOI https://doi.org/10.2147/OTT.S182225
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Sanjeev Srivastava
Backgrounds: Cholangiocarcinoma
(CCA) is epithelial cell malignancy with very poor prognosis. A lot of patients
were diagnosed at advanced stage of CCA and no risk factors were identified.
There are limited treatment options available for the management of CCA
patients. It is urgent to develop effective targeted therapies for the
treatment of CCA. miRNAs are small noncoding RNAs that negatively regulate the
target genes. In this study, we investigated the role and mechanism of
miR-10a-5p in CCA.
Methods: Human CCA cell lines (CCLP1 and SG-231) were transfected with miR-10a-5p mimic or miR-10a-5p inhibitor. qRT-PCR was performed to detect the miR-10a-5p level. Proliferation, colony formation, and apoptosis were analyzed. Luciferase reporter assay was used to explore the targeting of miR-10a-5p on PTEN. For in vivo tumorigenesis assay, CCLP1 cells with stable knockdown of miR-10a-5p or control CCLP1 cells were injected subcutaneously into the flank of the SCID mice and animals were monitored for tumor growth.
Results: miR-10a-5p expression was significantly upregulated in human CCA cell lines (CCLP1 and SG-231). Inhibition of miR-10a-5p significantly suppressed the proliferation and induced apoptosis in CCLP1 and SG-231. PTEN is a direct target of miR-10a-5p in CCA cells.
Conclusion: Inhibition of miR-10a-5p can decrease CCA cells growth by downregulation of Akt pathway. These results indicate that miR-10a-5p may serve as a potential target for the treatment of CCA and help to develop effective therapeutic strategies.
Keywords: miR-10a-5p, cholangiocarcinoma, PTEN, Akt, liver, proliferation
Methods: Human CCA cell lines (CCLP1 and SG-231) were transfected with miR-10a-5p mimic or miR-10a-5p inhibitor. qRT-PCR was performed to detect the miR-10a-5p level. Proliferation, colony formation, and apoptosis were analyzed. Luciferase reporter assay was used to explore the targeting of miR-10a-5p on PTEN. For in vivo tumorigenesis assay, CCLP1 cells with stable knockdown of miR-10a-5p or control CCLP1 cells were injected subcutaneously into the flank of the SCID mice and animals were monitored for tumor growth.
Results: miR-10a-5p expression was significantly upregulated in human CCA cell lines (CCLP1 and SG-231). Inhibition of miR-10a-5p significantly suppressed the proliferation and induced apoptosis in CCLP1 and SG-231. PTEN is a direct target of miR-10a-5p in CCA cells.
Conclusion: Inhibition of miR-10a-5p can decrease CCA cells growth by downregulation of Akt pathway. These results indicate that miR-10a-5p may serve as a potential target for the treatment of CCA and help to develop effective therapeutic strategies.
Keywords: miR-10a-5p, cholangiocarcinoma, PTEN, Akt, liver, proliferation
