已发表论文

中国南方败血症患者血液标本中常见可动遗传因子检测和多重耐药革兰阴性杆菌的基因分型

 

Authors Rui Y, Lu W, Li S, Cheng C, Sun J, Yang Q

Received 14 February 2018

Accepted for publication 2 June 2018

Published 12 October 2018 Volume 2018:11 Pages 1741—1750

DOI https://doi.org/10.2147/IDR.S165513

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 3

Editor who approved publication: Dr Eric Nulens

Background: Integron, ISCR1 and complex class 1 integrons lead bacteria to become resistant to antibiotic regimens. The aim of this study was to detect common mobile genetic elements of multidrug-resistant Gram-negative bacilli and evaluate the genotyping of these bacilli in blood specimens from septicemia patients in southern China.
Methods: A total of 837 Gram-negative bacilli including 578 strains containing Enterobacteriaceae  and 259 strains containing non-fermentative bacilli were investigated in blood samples collected from septicemia patients between 2011 and 2014 in southern China. Mobile genetic elements, such as class 1 integrons, the insertion sequence common region 1 (ISCR1), and complex class 1 integrons, were detected from the 837 strains. 
Results: Twenty-seven types of gene cassette arrays were found among 837 strains in which 492 (58.8%) class 1 integron-positive isolates and 254 (51.6%) gene cassette-positive isolates were found, including the first description of two types, aac A4-bla IMP-1-bla OXA-30-cat B3 and aac (6′)-II-aad A13-cml A8-bla OXA-10, in the corresponding species and two gene cassettes, putative helicase and aad A-like, originally detected in integrons. Twelve types of ISCR1-linked resistance gene regions in 196 ISCR1-positive bacilli and seven different types of complex class 1 integron-positive strains were obtained including four distinct complex class 1 integrons that have never been described in any species. Enterobacterial repetitive intergenic consensus (ERIC)-PCR fngerprinting showed that isolates with identical gene profles were clonally unrelated. 
Conclusion: Our results indicated that we should pay more attention to enhance the quality of infection control measures and prevent hospital infection, so as to avoid the outbreak of multidrug-resistant Gram-negative bacilli.
Keywords: septicemia, multidrug-resistant, integron, ISCR1 , complex class 1 integrons




Figure 2 Twelve distinct ISCR1-linked resistance gene arrangements.