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Authors Liu S, Liu Y, Lu Q, Zhou X, Chen L, Liang W
Received 11 March 2018
Accepted for publication 30 May 2018
Published 12 October 2018 Volume 2018:11 Pages 6845—6851
DOI https://doi.org/10.2147/OTT.S167900
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 3
Editor who approved publication: Dr William Cho
Purpose: Epithelial ovarian cancer (EOC) is among the most common malignant
tumors of the endocrine system. Numerous studies have shown that genetic
factors are important in the development of EOC, and there is evidence that
long noncoding RNA molecules (lncRNAs) can regulate gene expression at the
transcription, posttranscription, and epigenetic levels to influence cancer
proliferation and invasion, cell differentiation, and apoptosis. However, the
roles of lncRNAs in the pathogenesis of EOC remain unclear. Here, we
investigated the role of the lncRNA, taurine upregulated gene 1 (TUG1 ), in EOC.
Patients and methods: TUG1 mRNA levels were
evaluated in EOC and matched normal tissue samples and in EOC cell lines by
quantitative real-time PCR. Lentiviral vectors expressing the lncRNA, TUG1 , and siRNA targeting TUG1 were constructed and
transfected into EOC cells. MTT and Transwell assays were used to determine the
effects of TUG1 on cell proliferation,
migration, and invasion. Western blotting was performed to determine the
influence of TUG1 up- or
downregulation on WNT/β-catenin signaling, which is involved in the occurrence
and development of cancer.
Results: TUG1 expression was clearly
elevated in EOC compared with control tissue and cells. Moreover, TUG1 expression was
associated with lymphatic metastasis, T stage, and clinical stage in patients
with EOC. Downregulation of TUG1 in EOC
inhibited cell proliferation, migration, and invasion. In EOC cells, levels of
the WNT/β-catenin pathway factors, β-catenin, cyclin D1, and c-Myc, were significantly
up- and downregulated in response to TUG1 over- and
underexpression, respectively.
Conclusion: Our data suggest that knockdown of TUG1 may represent a novel
therapeutic approach for the management of EOC.
Keywords: epithelial ovarian cancer, long noncoding RNA, prognosis,
molecular mechanisms