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Authors Lin Y, Meng F, Lu Z, Chen K, Tao Y, Ouyang Y, Cao X
Received 25 June 2018
Accepted for publication 9 August 2018
Published 4 October 2018 Volume 2018:10 Pages 4191—4202
DOI https://doi.org/10.2147/CMAR.S178219
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Antonella D'Anneo
Background: Pyruvate kinase isozyme type M2 (PKM2) is a key glycolytic enzyme
and is upregulated in multiple human malignancies. However, the role of PKM2 in
human cervical cancer (CC) remains elusive. Thus, this study explored the role
of PKM2 in CC by detecting its expression patterns in human CC tissues and cell
lines and investigated its effects on cell proliferation and invasion.
Materials and
methods: Quantitative reverse transcription
polymerase chain reaction (qRT-PCR), immunohistochemistry and western blotting
assays were used to detect the expression of PKM2 in CC tissues and CC cells.
In vitro, we overexpressed and knocked down PKM2 expression in CC cell lines
and investigated the biological function and underlying mechanism of PKM2 in
cervical carcinogenesis.
Results: The results showed that PKM2 mRNA and protein were highly
expressed in CC tissues and cell lines. Furthermore, increasing PKM2 expression
was closely correlated with the clinical stage (P =0.001)
and lymph node metastasis (P =0.023). The
functional roles of PKM2 were determined using Cell Counting Kit-8, colony
formation, and transwell assays. The results showed that PKM2 knockdown
inhibited cell proliferation and the migratory and invasive capacities of CC
cells, suppressed epithelial–mesenchymal transition (EMT), and inhibited
Wnt/β-catenin signaling in vitro. However, overexpression of PKM2 led to
increased proliferation and invasion activity as well as the EMT in CC
cells.
Conclusion: Taken together, our study results revealed that PKM2 may act as a
molecular target for CC treatment.
Keywords: Cervical cancer, PKM2, invasion, epithelial–mesenchymal
transition, migration