已发表论文

九里香抑制肿瘤细胞源性培养基诱导的血管生成并抑制斑马鱼和内皮细胞中的 AKT 活化

 

Authors Long W, Wang M, Luo X, Huang G, Chen J

Received 10 July 2017

Accepted for publication 14 May 2018

Published 24 September 2018 Volume 2018:12 Pages 3107—3115

DOI https://doi.org/10.2147/DDDT.S145956

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 2

Editor who approved publication: Dr Sukesh Voruganti

Introduction: Lung cancer is a major cancer type and a leading cause of cancer-related death. Angiogenesis plays a crucial role in lung cancer pathogenesis and its inhibition is beneficial to patients.
Materials and methods: Murrangatin, a natural product, can inhibit the proliferation of lung cancer cells, so herein we investigated its anti-angiogenic effects in transgenic zebrafish TG (fli1: EGFP) and in lung cancer cell-induced angiogenesis in human umbilical vein endothelial cells. 
Results: We found that murrangatin strongly inhibited the growth of subintestinal vessels in zebrafish embryos and tumor conditioned media-induced angiogenic phenotypes including cell proliferation, cell invasion, cell migration, and tube formation. Additionally, murrangatin greatly attenuated conditioned medium-induced AKT phosphorylation, but not extracellular signal-regulated kinase 1/2 phosphorylation.
Discussion and conclusion: These findings indicate that murrangatin can inhibit tumor-induced angiogensis, at least in part through the regulation of AKT signaling pathways. Murrangatin may, therefore, be a potential candidate for the development of new anti-lung-cancer drugs.
Keywords: anti-angiogenesis, murrangatin, conditioned medium, zebrafish, HUVECs, AKT




Figure 2 Effects of murrangatin on morphological changes of zebrafish embryo...