Objectives: MUTYH  is a protein-coding gene that takes part in base excision repair. Many previous studies have reported that MUTYH  is directly related to hereditary adenomatous polyposis and colorectal cancer and is also associated with other cancers. However, the relationship between MUTYH  and bladder cancer (BC) is unknown.
Materials and methods: The expression of MUTYH  and clinical characteristics of BC were collected from databases including The Cancer Genome Atlas and Cancer Cell Line Encyclopedia. RNA sequencing and quantitative real-time PCR were used to detect MUTYH  expression in SW780 BC cells. The level of MUTYH  was stably downregulated by lentivirus-mediated vector in SW780 cells. Cell proliferation was evaluated using Cell Counting Kit-8 assay and 5-ethynyl-20-deoxyuridine assay, migration was detected using scratch assay and Transwell assay, and apoptosis was determined using ELISA.
Results: MUTYH  was upregulated in BC tissues and SW780 cells and its expression level was positively associated with the stage and grade of carcinomas. MUTYH  was successfully downregulated in SW780 cells by transducing with a lentivirus-mediated shRNA targeting MUTYH . MUTYH  knockdown inhibited the proliferation and migration and induced apoptosis in SW780 cells.
Conclusion: Our data suggest that MUTYH  is a new participant in bladder urothelial carcinoma. MUTYH  may play a role as a biomarker and therapeutic target in BC.
Keywords: MUTYH , bladder cancer, cell proliferation, migration, apoptosis