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已发表论文

三维结构金纳米粒子电极的敏感电化学免疫

 

Authors Zhong G, Lan R, Zhang W, Fu F, Sun Y, Peng H, Chen T, Cai Y, Liu A, Lin J, Lin X

Published Date March 2015 Volume 2015:10 Pages 2219—2228

DOI http://dx.doi.org/10.2147/IJN.S76200

Received 22 October 2014, Accepted 14 January 2015, Published 19 March 2015

Abstract: A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP) based on a three-dimensional nanostructure gold electrode using a facile, rapid, “green” square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were characterized by scanning electron microscopy and cyclic voltammetry. A “sandwich-type” detection strategy using an electrochemical immunosensor was employed. Under optimal conditions, a good linear relationship between the current response signal and the AFP concentrations was observed in the range of 10–50 ng/mL with a detection limit of 3 pg/mL. This new immunosensor showed a fast amperometric response and high sensitivity and selectivity. It was successfully used to determine AFP in a human serum sample with a relative standard deviation of <5% (n=5). The proposed immunosensor represents a significant step toward practical application in clinical diagnosis and monitoring of prognosis.
Keywords: electrochemical immunosensors, three-dimensional nanostructure gold electrode, square-wave oxidation-reduction cycle, alpha-fetoprotein






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