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具有中孔大小和孔隙体积的介孔钙硅凝胶通过加载和持续释放 rhBMP-2 而对 hMSC 的行为产生影响

 

Authors Song W, Li X, Qian J, Lv G, Yan Y, Su J, Wei J

Published Date March 2015 Volume 2015:10 Pages 1715—1726

DOI http://dx.doi.org/10.2147/IJN.S70934

Received 10 July 2014, Accepted 27 August 2014, Published 4 March 2015

Abstract: Mesoporous calcium–silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm3/g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium–silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

Keywords: mesoporous calcium–silicon xerogels, pore size, pore volume, load-release, rhBMP-2


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