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Authors Chen DW, Zhang CY, Lin JM, Song XY, Wang HY
Received 27 February 2018
Accepted for publication 3 April 2018
Published 18 May 2018 Volume 2018:10 Pages 1275—1283
DOI https://doi.org/10.2147/CMAR.S166740
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Jaya Mallela
Peer reviewer comments 2
Editor who approved publication: Dr Antonella D'Anneo
Aim: The aim of this study was to analyze the diagnostic and prognostic
values of the circular RNA (circRNA) hsa_circ_0128298 in hepatocellular
carcinoma (HCC).
Patients and
methods: The global circRNA expression was
measured using circRNA microarray using three pairs of cancer and noncancerous
tissues from HCC patients. The microarray analysis revealed that two circRNAs
were differentially expressed in the three pairs of cancerous and noncancerous
tissues. The higher levels of two representative circRNAs, such as
hsa_circ_0128298 and hsa_circ_0091582, were further confirmed by real-time
polymerase chain reaction. In addition, the association between the expression
level of hsa_circ_0128298 and the clinicopathological features of patients with
HCC was further analyzed. The clinical diagnosis value was confirmed by
receiver operating characteristic (ROC) curve analysis. Independent prognostic
factors of patient outcome were identified using the Cox regression model. The
survival data were analyzed by the Kaplan–Meier method, and the differences
were evaluated using log-rank tests. Two-sided P-values
<0.05 were considered statistically significant.
Results: The expression levels of hsa_circ_0128298 in HCC were
significantly higher than those of paratumorous tissues (P <0.001). Additionally,
hsa_circ_0128298 was a diagnostic factor, with the area under the ROC curve of
0.668 (95% CI =0.503–0.794, P <0.001). The
sensitivity and specificity values were 0.716 and 0.815, respectively. The AFP
and hsa_circ_0128298 expression levels were independent prognostic factors. The
overall survival of patients with low hsa_circ_0128298 expression was
significantly higher than that of patients with high hsa_circ_0128298
expression.
Conclusion: hsa_circ_0128298 may promote proliferation and metastasis and
potentially represents a novel diagnostic and prognostic biomarker for HCC
patients. However, studies with larger sample size are needed to confirm our
conclusion.
Keywords: hepatocellular carcinoma, circular RNA, hsa_circ_0128298,
biomarker, diagnosis, prognosis