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Authors Zhang R, Chen B, Tong X, Wang Y, Wang C, Jin J, Tian P, Li W
Received 3 January 2018
Accepted for publication 8 March 2018
Published 16 May 2018 Volume 2018:10 Pages 1209—1218
DOI https://doi.org/10.2147/CMAR.S161382
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 4
Editor who approved publication: Dr Leylah Drusbosky
Objectives: Although
different methods have been established to detect epidermal growth factor
receptor (EGFR) T790M mutation in circulating tumor DNA (ctDNA), a wide range
of diagnostic accuracy values were reported in previous studies. The aim of
this meta-analysis was to provide pooled diagnostic accuracy measures for
droplet digital PCR (ddPCR) in the diagnosis of EGFR T790M mutation based on
ctDNA.
Materials and
methods: A systematic review and meta-analysis
were carried out based on resources from Pubmed, Web of Science, Embase and
Cochrane Library up to October 11, 2017. Data were extracted to assess the
pooled sensitivity, specificity, positive likelihood ratio, negative likelihood
ratio (NLR), diagnostic OR (DOR), and areas under the summary
receiver-operating characteristic curve (SROC).
Results: Eleven of 311 studies identified have met the including criteria.
The sensitivity and specificity of ddPCR for the detection of T790M mutation in
ctDNA ranged from 0.0% to 100.0% and 63.2% to 100.0%, respectively. For the
pooled analysis, ddPCR had a performance of 70.1% (95% CI, 62.7%–76.7%)
sensitivity, 86.9 % (95% CI, 80.6%–91.7%) specificity, 3.67 (95% CI, 2.33–5.79)
PLR, 0.41 (95% CI, 0.32–0.55) NLR, and 10.83 (95% CI, 5.86–20.03) DOR, with the
area under the SROC curve being 0.82.
Conclusion: The ddPCR harbored a good performance for detection of EGFR T790M
mutation in ctDNA.
Keywords: T790M, droplet digital PCR, circulating tumor DNA, lung cancer