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Authors Guo S, Zhang J, Wang B, Zhang B, Wang X, Huang L, Liu H, Jia B
Received 9 October 2017
Accepted for publication 20 March 2018
Published 8 May 2018 Volume 2018:11 Pages 2603—2614
DOI https://doi.org/10.2147/OTT.S153535
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Amy Norman
Peer reviewer comments 4
Editor who approved publication: Dr Ingrid Espinoza
Objective: The study aimed to screen microRNAs (miRNAs) that can be used for the
early detection of colorectal cancer (CRC) based on differential expression of
miRNA in serum.
Materials and methods: A three-stage study was designed with a total of
217 CRCs, 168 colorectal adenomas (CRAs), and 190 healthy controls (HCs). A
quantitative reverse transcription polymerase chain reaction was performed in
three stages. We screened 528 miRNA expression profiles in the sera of 40 patients
(CRC n=20, CRA n=10, and HC n=10) for candidate miRNAs, then 210 serum samples
(CRC n=90, CRA n=60, and HC n=60) were used for screening of candidate miRNAs.
Three hundred and twenty-five independent individual samples (CRC n=107, CRA
n=98, and HC n=120) were used to validate the most differentially-expressed
miRNAs in the screening stage, and binary logistic regression was used in the
validation stage. A receiver operating characteristic curve was drawn to
evaluate the diagnostic accuracy.
Results: A 5-serum miRNA panel (miRNA-1246, miRNA-202-3p,
miRNA-21-3p, miRNA-1229-3p, and miRNA-532-3p) effectively distinguished CRCs
from HCs with 91.6% sensitivity and 91.7% specificity. The area under the curve
(AUC) was 0.960 (95% confidence interval [CI]: 0.937–0.983). In addition, the
panel also accurately distinguished CRCs from CRAs with 94.4% sensitivity and
84.7% specificity. The AUC was 0.951 (95% CI: 0.922–0.980).
Conclusion: Our 5-serum miRNA panel accurately distinguished
CRCs from CRAs and HCs with high sensitivity and specificity. The 5-serum miRNA
panel may be a promising prospect for application as a nonintrusive and
inexpensive method for the early detection of CRC.
Keywords: colorectal
cancer, colorectal adenoma, miRNA