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Authors Zhang S, Huang S, Lu L, Song X, Li P, Wang F
Received 30 November 2017
Accepted for publication 28 February 2018
Published 18 April 2018 Volume 2018:13 Pages 2377—2394
DOI https://doi.org/10.2147/IJN.S158536
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Jiang Yang
Peer reviewer comments 2
Editor who approved publication: Dr Linlin Sun
Introduction: The development of ideal vaccine adjuvants for intranasal
vaccination can provide convenience for many vaccinations. As an ideal
intranasal vaccine adjuvant, it should have the properties of assisting soluble
antigens to pass the mucosal barrier and potentiating both systemic and mucosal
immunity via nasal administration.
Methods: By using the advantages of polysaccharides, which can promote both
T-helper 1 and 2 responses, curdlan sulfate (CS)–O -(2-hydroxyl)propyl-3-trimethyl
ammonium chitosan chloride (O -HTCC)
nanoparticles were prepared by interacting CS with O -HTCC,
and the adjuvancy of the nanoparticles was investigated.
Results: The results showed that the polysaccharide-based nanoparticles induced
the proliferation and activation of antigen-presenting cells. High
protein-loading efficiency was obtained by testing with the model antigen
ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O -HTCC complexes was taken up
easily by the epithelium. To evaluate the capacity of the Ova/CS/O -HTCC nanoparticles for immune
enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal
lavage fluid from intranasally vaccinated mice. The results showed that Ova/CS/O -HTCC nanoparticles induced
activation and maturation of antigen-presenting cells and provoked the
proliferation and differentiation of lymphocytes more significantly compared to
the immunization of Ova mixed with aluminum hydroxide gel. Furthermore, CS/O -HTCC evoked a significantly
higher level of Ova-specific antibodies.
Conclusion: Therefore, these results suggest that CS/O -HTCC
nanoparticles are ideal vaccine adjuvants for soluble antigens used in
intranasal or mucosal vaccination.
Keywords: curdlan sulfate, O -linked
quaternized chitosan, nasal mucosa immunization, nanoparticle, adjuvant,
ovalbumin, immune response