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Authors Yu X, He X, Yang T, Zhao L, Chen Q, Zhang S, Chen J, Xu J
Received 13 November 2017
Accepted for publication 15 February 2018
Published 17 April 2018 Volume 2018:13 Pages 2337—2347
DOI https://doi.org/10.2147/IJN.S156932
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Govarthanan Muthusamy
Peer reviewer comments 2
Editor who approved publication: Dr Lei Yang
Background: Dopamine (DA) is an important neurotransmitter in the hypothalamus
and pituitary gland, which can produce a direct influence on mammals’ emotions
in midbrain. Additionally, the level of DA is highly related with some
important neurologic diseases such as schizophrenia, Parkinson, and
Huntington’s diseases, etc. In light of the important roles that DA plays in
the disease modulation, it is of considerable significance to develop a
sensitive and reproducible approach for monitoring DA.
Purpose: The objective of this study was to develop an
efficient approach to quantitatively monitor the level of DA using Ag
nanoparticle (NP) dimers and enhanced Raman spectroscopy.
Methods: Ag NP dimers were synthesized for the sensitive
detection of DA via surface-enhanced Raman scattering (SERS). Citrate was used
as both the capping agent of NPs and sensing agent to DA, which is
self-assembled on the surface of Ag NP dimers by reacting with the surface
carboxyl group to form a stable amide bond. To improve accuracy and precision,
the multiplicative effects model for surface-enhanced Raman spectroscopy was
utilized to analyze the SERS assays.
Results: A low limits of detection (LOD) of 20 pM and a
wide linear response range from 30 pM to 300 nM were obtained for DA
quantitative detection. The SERS enhancement factor was theoretically valued at
approximately 107 by discrete dipole
approximation. DA was self-assembled on the citrate capped surface of Ag NPs
dimers through the amide bond. The adsorption energy was estimated to be 256
KJ/mol using the Langmuir isotherm model. The density functional theory was
used to simulate the spectral characteristics of SERS during the adsorption of
DA on the surface of the Ag dimers. Furthermore, to improve the accuracy and
precision of quantitative analysis of SERS assays with a multiplicative effects
model for surface-enhanced Raman spectroscopy.
Conclusion: A LOD of 20 pM DA-level was obtained, and the
linear response ranged from 30 pM to 300 nM for quantitative DA detection. The
absolute relative percentage error was 4.22% between the real and predicted DA
concentrations. This detection scheme is expected to have good applications in
the prevention and diagnosis of certain diseases caused by disorders in the DA
level.
Keywords: surface-enhanced
Raman scattering, SERS, multiplicative effects model for surface-enhanced Raman
spectroscopy, MEMSERS, dopamine detection, Ag NP dimers