论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
Authors Chen G, Xie Y
Received 22 September 2017
Accepted for publication 31 December 2017
Published 5 April 2018 Volume 2018:11 Pages 1909—1920
DOI https://doi.org/10.2147/OTT.S152362
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Narasimha Reddy Parine
Peer reviewer comments 2
Editor who approved publication: Dr Jianmin Xu
Background: Amounting evidence indicate that miRNAs play an important role in the
development of various cancers. MiR-495 is a potential tumor suppressor in
cancers, however its role in melanoma is still elusive. The study aimed to
investigate the role of miR-495 and the underlying mechanisms in melanoma
cells.
Methods: The levels of miR-495 in melanoma tissues and cell lines were
measured by quantitative real-time polymerase chain reaction. Mimics of miR-495
was transfected into human melanoma cells A375 and MeWo. Cell viability of
miR-495-transfected cells was assayed by MTT assay. Cell migration and invasion
of miR-495 transfected cells were measured by wound healing assay and transwell
assay, respectively. Nucleosome enzyme-linked immunosorbent assay was performed
to measure the apoptosis induced by overexpression of miR-495. Luciferase
reporter assays were performed to verify the interaction between miR-495 and
its target PBX3.
Results: It was found that the expression levels of miR-495 were
down-regulated in melanoma tissues and cells. Moreover, overexpression of
miR-495 inhibited melanoma cell proliferation, migration and invasion in vitro.
PBX3 was identified as a target for inhibition by miR-495 and was confirmed by
luciferase assay, quantitative real-time polymerase chain reaction and western
blot. We also indicated that silencing of PBX3 also repressed melanoma cell
proliferation, migration and invasion in vitro.
Conclusion: In summary, our findings demonstrated that miR-495 functions as a tumor
suppressor in human melanoma via directly targeting PBX3.
Keywords: melanoma, miR-495, PBX3, cell migration, cell invasion