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Authors Wang G, Li L, Liu B, Han X, Wang CH, Wang JW
Received 10 August 2017
Accepted for publication 25 October 2017
Published 4 April 2018 Volume 2018:11 Pages 1849—1859
DOI https://doi.org/10.2147/OTT.S148776
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Carlos Vigil Gonzales
Purpose: This study aimed to explore significant genes and pathways involved in
the pathogenesis of supratentorial primitive neuroectodermal tumor (sPNET).
Materials and
methods: Gene expression profile of
GSE14295 was downloaded from publicly available Gene Expression Omnibus (GEO)
database. Differentially expressed genes (DEGs) were screened out in primary
sPNET samples compared with normal fetal and adult brain reference samples
(sPNET vs fetal brain and sPNET vs adult brain). Pathway enrichment analysis of
these DEGs was conducted, followed by protein–protein interaction (PPI) network
construction and significant module selection. Additionally, transcription
factors (TFs) regulating the common DEGs in the two comparison groups were
identified, and the regulatory network was constructed.
Results: In total, 526 DEGs (99 up- and 427 downregulated) in sPNET vs fetal brain
and 815 DEGs (200 up- and 615 downregulated) in sPNET vs adult brain were
identified. DEGs in sPNET vs fetal brain and sPNET vs adult brain were
associated with calcium signaling pathway, cell cycle, and p53 signaling
pathway. CDK1 , CDC20 , BUB1B , and BUB1 were hub nodes in the
PPI networks of DEGs in sPNET vs fetal brain and sPNET vs adult brain.
Significant modules were extracted from the PPI networks. In addition, 64
upregulated and 200 downregulated overlapping DEGs were identified in both
sPNET vs fetal brain and sPNET vs adult brain. The genes involved in the
regulatory network upon overlapping DEGs and the TFs were correlated with
calcium signaling pathway.
Conclusion: Calcium signaling pathway and several genes (CDK1 , CDC20 , BUB1B , and BUB1 ) may play important roles in
the pathogenesis of sPNET.
Keywords: primitive neuroectodermal tumor, microarray analysis,
protein-protein interaction, transcription factors