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Authors Gao X, Zhang G, Huang L
Received 13 July 2017
Accepted for publication 31 October 2017
Published 15 March 2018 Volume 2018:11 Pages 1447—1456
DOI https://doi.org/10.2147/OTT.S146300
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Manfred Beleut
Peer reviewer comments 3
Editor who approved publication: Dr William Cho
Objective: The objective of this paper was to assess the effects of hydatid cyst
fluid (HCF) of Echinococcus granulosus on
melanoma A375 cell proliferation and apoptosis.
Methods: A375 cells were classified into five groups by in vitro culture:
normal group, control group, 10% HCF group, 20% HCF group and 30% HCF group.
Trypan blue staining method was employed to detect the toxicity of HCF. Effects
of different concentrations of HCF on melanoma A375 cell proliferation at
different time points were evaluated using the
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Flow
cytometry and propidium iodide (PI) staining were used to detect cell cycle,
and Annexin-V/PI double staining method was used to determine A375 cell
apoptotic rate. Western blotting was applied to detect the expression of
phosphorylated extracellular regulated protein kinases, proliferating cell
nuclear antigen (PCNA), cell-cycle-related proteins (cyclin A, cyclin B1,
cyclin D1 and cyclin E) and apoptosis-related proteins (Bcl-2, Bax and
caspase-3).
Results: HCF with a high concentration was considered as atoxic to A375 cells.
HCF promoted A375 cell proliferation, and the effects got stronger with an
increase in concentrations but was retarded after reaching a certain range of
concentrations. HCF increased phosphorylation level and expression of
extracellular regulated protein kinase, as well as PCNA expression. HCF also
promoted the transferring progression of A375 cells from the G0/G1 phase to the
S phase to increase the cell number in S phase and increased the expression of
cyclin A, cyclin D1 and cyclin E. HCF increased the expression of procaspase-3
(the precursor of apoptosis-related protein caspase-3) and antiapoptotic
protein-Bcl-2, and decreased the expression of proapoptotic factor Bax, thereby
inhibiting cell apoptosis.
Conclusion: As a result, this study confirmed that HCF promotes proliferation
and inhibits apoptosis of melanoma A375 cells.
Keywords: hydatid cyst fluid, melanoma, A375 cell, proliferation, apoptosis, cell
cycle
* 在 1447 页,通讯作者的联系信息应该如下:
Correspondence: Li Huang
Department of General Surgery, Shanghai General Hospital, No 100, Haining Road, Shanghai 200080, China
Tel/fax +86 21 6324 0090 3136
Email anorlee@163.com
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