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Authors Mo Z, Hu MG, Yu F, Shao LJ, Fan KX, Jiao SC
Received 23 November 2017
Accepted for publication 16 January 2018
Published 5 March 2018 Volume 2018:11 Pages 1215—1222
DOI https://doi.org/10.2147/OTT.S157914
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Amy Norman
Peer reviewer comments 2
Editor who approved publication: Dr Ingrid Espinoza
Background: Leukemia related protein 16 (LRP16), one of the genes belonging to the
macro domain family, has been found up-regulated in various tumors including
testicles, ovaries and mucosa of colon and is associated with poor clinical
outcomes.
Purpose: The objective of this study was to investigate expression pattern
and biological roles of LRP16 in pancreatic cancer.
Patients and
methods: Western blot and
immunohistochemistry were used to investigate the expression of LRP16 in
pancreatic cancer cell lines and tissues. qRT-PCR was utilized to examine LRP16
mRNA expression. Lentivirus based overexpression and knockdown of LRP16 was
carried out in four pancreatic cancer cell lines (Panc1, CFPAC1, SW1990 and
AsPC1). Cell proliferation, migration and invasion were determined by MTS and
transwell assay, respectively. Flow cytometry was performed to investigate cell
apoptosis. In vivo, the tumorigenic ability of LRP16 was determined in a
NOD/SCID mouse model.
Results: In the present study, we found that LRP16 expression was increased in
pancreatic tumor samples, compared with normal tissues. Moreover, the LRP16
expression was positive in 60.9% of 156 specimens and correlated with tumor
size, clinical stage, distant metastasis and tumor differentiation.
Multivariate Cox regression analysis revealed that the level of LRP16
expression was an independent prognostic factor for overall survival in
pancreatic cancer patients. Furthermore, silencing of LRP16 significantly
accelerated apoptosis, decrease proliferation, migration and invasion of
pancreatic cancer cell lines in vitro. In contrast, overexpression of LRP16
attenuated apoptosis, promoted proliferation, migration and invasion. In
addition, in vivo study revealed that down regulation of LRP16 could attenuate
tumor growth and prolong the survival. On the contrary, up-regulation of LRP16
could promote tumor growth and shorten their survival.
Conclusion: These findings suggest that LRP16 played an oncogenic role in
pancreatic carcinoma.
Keywords: leukemia-related protein 16, pancreatic cancer, proliferation, metastasis