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Authors Wang CW, Gu B, Liu QQ, Pang YF, Xiao R, Wang SQ
Received 30 August 2017
Accepted for publication 7 December 2017
Published 27 February 2018 Volume 2018:13 Pages 1159—1178
DOI https://doi.org/10.2147/IJN.S150336
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Alexander Kharlamov
Peer reviewer comments 4
Editor who approved publication: Dr Linlin Sun
Background: Pathogenic bacteria have always been a significant threat to human
health. The detection of pathogens needs to be rapid, accurate, and convenient.
Methods: We present a sensitive surface-enhanced Raman scattering (SERS)
biosensor based on the combination of vancomycin-modified Ag-coated magnetic
nanoparticles (Fe3O4@Ag-Van MNPs) and Au@Ag
nanoparticles (NPs) that can effectively capture and discriminate bacterial
pathogens from solution. The high-performance Fe3O4@Ag MNPs were modified
with vancomycin and used as bacteria capturer for magnetic separation and
enrichment. The modified MNPS were found to exhibit strong affinity with a
broad range of Gram-positive and Gram-negative bacteria. After separating and
rinsing bacteria, Fe3O4@Ag-Van MNPs and Au@Ag
NPs were synergistically used to construct a very large number of hot spots on
bacteria cells, leading to ultrasensitive SERS detection.
Results: The dominant merits of our dual enhanced strategy included high
bacterial-capture efficiency (>65%) within a wide pH range (pH 3.0–11.0), a
short assay time (<30 min), and a low detection limit (5×102 cells/mL). Moreover, the spiked tests show that this method is
still valid in milk and blood samples. Owing to these capabilities, the
combined system enabled the sensitive and specific discrimination of different
pathogens in complex solution, as verified by its detection of Gram-positive
bacterium Escherichia coli , Gram-positive
bacterium Staphylococcus aureus , and
methicillin-resistant S. aureus .
Conclusion: This method has great potential for field applications in food
safety, environmental monitoring, and infectious disease diagnosis.
Keywords: surface-enhanced Raman scattering, Fe3O4@Ag magnetic
nanoparticle, Au@Ag nanoparticles, vancomycin-modified, rapid bacteria
detection