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Authors Zheng SS, Qin T, Lu Y, Huang YF, Luo L, Liu ZG, Bo RN, Hu YL, Liu JG, Wang DY
Received 9 October 2017
Accepted for publication 7 December 2017
Published 26 January 2018 Volume 2018:13 Pages 569—583
DOI https://doi.org/10.2147/IJN.S153567
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Govarthanan Muthusamy
Peer reviewer comments 2
Editor who approved publication: Dr Linlin Sun
Background: Poly lactide (PLA) was proved in the last years to be good for use in
sustained drug delivery and as carriers for vaccine antigens. In our previous
research, pachyman (PHY)-encapsulated PLA (PHYP) nanospheres were synthesized
and their function of controlling drug release was demonstrated.
Purpose: In order to modify the fast drug-release rate of PHY
when inoculated alone, the maturation of bone marrow dendritic cells (BMDCs) in
vitro and their immunological enhancement in vivo were explored using PHYP
nanospheres.
Methods: The maturation and antigen uptake of BMDCs were
evaluated, both alone and with formulated antigen PHYP nanospheres, ie,
ovalbumin (OVA)-loaded PHYP nanospheres, as an antigen delivery system, to
investigate antigen-specific humoral and cellular immune responses.
Results: The results indicated that, when stimulated by
PHYP, the BMDCs matured as a result of upregulated expression of co-stimulatory
molecules; the mechanism was elucidated by tracing fluorescently labeled
antigens in confocal laser scanning microscopy images and observing the uptake
of nanospheres by transmission electron microscopy. It was further revealed
that mice inoculated with OVA-PHYP had augmented antigen-specific IgG
antibodies, increased cytokine secretion by splenocytes, increased splenocyte
proliferation, and activation of cluster of differentiation (CD)4+ and CD8+ T cells in
vivo. Elevated immune responses were produced by OVA-PHYP, possibly owing to
the activation and maturation of dendritic cells (in draining lymph nodes).
Conclusion: It was corroborated that PHY- and/or
OVA-encapsulated PLA nanospheres elicited prominent antigen-presenting effects
on BMDCs and heightened humoral and cellular immune responses compared with
other formulations.
Keywords: PHYP, bone
marrow dendritic cell, antigen delivery system, immune response