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Authors Li J, Huang YY, Deng XJ, Luo ML, Wang XF, Hu HY, Liu CD, Zhong M
Received 24 August 2017
Accepted for publication 31 October 2017
Published 18 January 2018 Volume 2018:11 Pages 427—440
DOI https://doi.org/10.2147/OTT.S149908
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Yao Dai
Abstract: Ovarian cancer is a
gynecological malignant tumor with a high mortality rate among women, owing to
metastatic progression and recurrence. Acquisition of invasiveness is
accompanied by the loss of epithelial features and a gain of a mesenchymal
phenotype, a process known as epithelial–mesenchymal transition (EMT). Transforming
growth factor-β (TGF-β) has been implicated in the regulation of EMT. In the
present study, we aimed to investigate the role of long noncoding RNA H19 and
microRNA-370 (miR-370-3p) in TGF-β-induced EMT. Ovarian cancer cell lines
SKOV-3 and OVCAR3 were incubated with different concentrations of TGF-β, and
the results showed that TGF-β treatment upregulated H19 and downregulated
miR-370-3p. In addition, an H19 knockdown or miR-370-3p overexpression
suppressed TGF-β-induced EMT, while H19 overexpression or a miR-370-3p
knockdown promoted TGF-β-induced EMT. Mechanistically, H19 could directly bind
to miR-370-3p and effectively act as its competing endogenous RNA. Furthermore,
we demonstrated that this activity of H19 was involved in its promotion of TGF-β-induced
EMT. Thus, our results may provide novel insights into the process of
TGF-β-induced EMT.
Keywords: transforming
growth factor-β, long noncoding RNA H19, microRNA-370-3p, competing endogenous
RNA, epithelial–mesenchymal transition