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Authors Feng Y, Chen L, Luo Q, Wu M, Chen Y, Shi X
Received 15 November 2017
Accepted for publication 5 December 2017
Published 17 January 2018 Volume 2018:12 Pages 171—177
DOI https://doi.org/10.2147/DDDT.S157109
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Palas Chanda
Peer reviewer comments 2
Editor who approved publication: Dr Qiongyu Guo
Purpose: Recent evidence has shown the involvement of inflammation in the
development of diabetic peripheral neuropathy (DPN). MicroRNA-146a (miR-146a)
is closely involved in the inflammatory response. However, the role of miR-146a
in the inflammatory reaction in DPN has not been clarified. This study was
designed to explore the role of miR-146a in the regulation of inflammatory
responses in DPN.
Methods: Rats were randomly divided into three groups
(n=6 per group): control group, type 2 diabetes mellitus (T2DM) group and DPN
group. T2DM and DPN rats were intraperitoneally injected with streptozotocin.
Sciatic nerve conduction velocity (NCV) was determined at the 6th week and the
12th week in each group. The expression of microRNAs was detected by
quantitative real-time polymerase chain reaction in three sciatic nerves for
each group of rats. Expression of inflammatory cytokines in nerve tissues and
plasma was measured by Western blot and Bio-Plex Pro™ assays.
Results: The NCV and expression levels of miR-146a in the
DPN group were significantly decreased (P <0.01) compared
to the other two groups. Expression of tumor necrosis factor alpha (TNF-α),
interleukin 1 beta (IL-1β) and nuclear factor kappa-light-chain-enhancer of
activated B cells (NF-κB) in the DPN group was significantly increased compared
with the control and T2DM groups (P <0.01).
Pearson’s correlation analysis showed that the expression level of miR-146a was
negatively correlated with the levels of IL-1β, TNF-α and NF-κB.
Conclusion: miR-146a is involved in the pathogenesis of DPN,
and its expression level is closely related to the inflammatory responses that
aggravate sciatic nerve injuries.
Keywords: type 2 diabetes
mellitus, diabetic peripheral neuropathy, microRNA-146a, NF-κB, cytokines