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对线粒体能量代谢肝细胞直接或通过一个库普弗细胞介导的胞外途径产生的二氧化硅纳米颗粒诱导损伤

 

Authors Xue Y, Chen Q, Ding T, Sun J

Published Date June 2014 Volume 2014:9(1) Pages 2891—2903

DOI http://dx.doi.org/10.2147/IJN.S60661

Received 14 January 2014, Accepted 22 February 2014, Published 12 June 2014

Abstract: The liver has been shown to be a primary target organ for SiO2 nanoparticles in vivo, and may be highly susceptible to damage by these nanoparticles. However, until now, research focusing on the potential toxic effects of SiO2 nanoparticles on mitochondria-associated energy metabolism in hepatocytes has been lacking. In this work, SiO2 nanoparticles 20 nm in diameter were evaluated for their ability to induce dysfunction of mitochondrial energy metabolism. First, a buffalo rat liver (BRL) cell line was directly exposed to SiO2 nanoparticles, which induced cytotoxicity and mitochondrial damage accompanied by decreases in mitochondrial dehydrogenase activity, mitochondrial membrane potential, enzymatic expression in the Krebs cycle, and activity of the mitochondrial respiratory chain complexes I, III and IV. Second, the role of rat-derived Kupffer cells was evaluated. The supernatants from Kupffer cells treated with SiO2 nanoparticles were transferred to stimulate BRL cells. We observed that SiO2 nanoparticles had the ability to activate Kupffer cells, leading to release of tumor necrosis factor-α, nitric oxide, and reactive oxygen species from these cells and subsequently to inhibition of mitochondrial respiratory chain complex I activity in BRL cells.
Keywords: SiO2 nanoparticles, mitochondria-associated energy metabolism, buffalo rat liver cells, Kupffer cells







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