已发表论文

氧化锌纳米粒子在人神经母细胞瘤 SHSY5Y 细胞中以尺寸依赖的方式诱导毒性反应

 

Authors Liu J, Kang Y, Yin S, Song B, Wei L, Chen L, Shao L

Received 14 August 2017

Accepted for publication 27 September 2017

Published 1 November 2017 Volume 2017:12 Pages 8085—8099

DOI https://doi.org/10.2147/IJN.S149070

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Alexander Kharlamov

Peer reviewer comments 2

Editor who approved publication: Dr Lei Yang

Abstract: Due to the widespread applications of zinc oxide nanoparticles (ZnO NPs), the potential exposure of workers, consumers, and scientists to these particles has increased. This potential for exposure has attracted extensive attention in the science community. Many studies have examined the toxicological profile of ZnO NPs in the immune system, digestive system, however, information regarding the toxicity of ZnO NPs in the nervous system is scarce. In this study, we detected the cytotoxicity of two types of ZnO NPs of various sizes – ZnOa NPs and ZnOb NPs – and we characterized the shedding ability of zinc ions within culture medium and the cytoplasm. We found that reactive oxygen species played a crucial role in ZnO NP-induced cytotoxicity, likely because zinc ions were leached from ZnO NPs. Apoptosis and cytoskeleton changes were also toxic responses induced by the ZnO NPs, and ZnOb NPs induced more significant toxic responses than ZnOa NPs in SHSY5Y cells. In conclusion, ZnO NPs induced toxic responses in SHSY5Y cells in a size-dependent manner, which can probably be attributed to their ion-shedding ability.
Keywords: zinc oxide, nanoparticles, SHSY5Y cells, reactive oxygen species, apoptosis, cell cytoskeleton