120017
论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
- 3.6 Breast Cancer (Dove Med Press)
- 4.3 Clin Epidemiol
- 2.6 Cancer Manag Res
- 3.2 Infect Drug Resist
- 4.1 Clin Interv Aging
- 6.1 Drug Des Dev Ther
- 4.1 Int J Chronic Obstr
- 8.7 Int J Nanomed
- 2.5 Int J Women's Health
- 3.2 Neuropsych Dis Treat
- 2.4 OncoTargets Ther
- 2.6 Patient Prefer Adher
- 2.6 Ther Clin Risk Manag
- 3.1 J Pain Res
- 3.5 Diabet Metab Synd Ob
- 4.5 Psychol Res Behav Ma
- 3.4 Nat Sci Sleep
- 2.4 Pharmgenomics Pers Med
- 2.6 Risk Manag Healthc Policy
- 4.6 J Inflamm Res
- 2.3 Int J Gen Med
- 3.9 J Hepatocell Carcinoma
- 3.3 J Asthma Allergy
- 2.5 Clin Cosmet Investig Dermatol
- 3.0 J Multidiscip Healthc
已发表论文
ω-3 多不饱和脂肪酸通过 JAK2/STAT3 信号通路调节巨噬细胞极化减轻术后肠麻痹
Authors Zhao X, Yu J, Zhang T, Zhang Y, Zhang L, Wang Y, Yi C, Pan H, Wang H, Ge M, Jiang Z, Wang G
Received 5 September 2025
Accepted for publication 9 February 2026
Published 13 February 2026 Volume 2026:19 562643
DOI https://doi.org/10.2147/JIR.S562643
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Alberto Caminero
Xuan Zhao,1,* Jing Yu,1,* Tianle Zhang,1 Yufei Zhang,1 Liuchuang Zhang,1 Ye Wang,1 Chen Yi,1 Huafeng Pan,2 Haifeng Wang,2 Miaomiao Ge,2 Zhiwei Jiang,2 Gang Wang2
1Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 2Department of General Surgery, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Gang Wang, Email gwang82@163.com Zhiwei Jiang, Email surgery34@163.com
Objective: This study explored the therapeutic effects and mechanisms of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) on postoperative ileus (POI) and their potential in preventing or treating POI.
Methods: A murine model of postoperative ileus (POI) and an in vitro macrophage inflammation model were employed. Analyses included the assessment of intestinal injury, motility, inflammatory cytokines, macrophage infiltration, and polarization. RNA sequencing implicated the JAK2/STAT3 pathway, which was validated through immunohistochemistry and Western blot. Subsequent STAT3 knockout and overexpression experiments in macrophages further elucidated the underlying mechanism.
Results: ω-3 PUFAs alleviated intestinal damage, restored motility, and reduced local pro-inflammatory cytokine levels in POI mice, along with reduced macrophage infiltration. In vitro, ω-3 PUFAs suppressed M1 polarization. RNA-seq implicated the JAK/STAT pathway, and further experiments confirmed that ω-3 PUFAs markedly suppressed the phosphorylation of JAK2, STAT3, and p65 in both macrophages and ileal tissue. Genetic manipulation of STAT3 established that ω-3 PUFAs attenuate inflammation primarily through modulating macrophage polarization.
Conclusion: ω-3 PUFAs alleviate POI by inhibiting the JAK2/STAT3 signaling pathway in macrophages, thereby suppressing their polarization toward the pro-inflammatory M1 phenotype and reducing local intestinal inflammation. These findings indicate that ω-3 PUFAs may be a promising prophylactic or therapeutic agent for POI.
Keywords: omega-3 polyunsaturated fatty acids, postoperative ileus, macrophage polarization, inflammatory cytokines, JAK2/STAT3 signaling pathway
1Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 2Department of General Surgery, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Gang Wang, Email gwang82@163.com Zhiwei Jiang, Email surgery34@163.com
Objective: This study explored the therapeutic effects and mechanisms of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) on postoperative ileus (POI) and their potential in preventing or treating POI.
Methods: A murine model of postoperative ileus (POI) and an in vitro macrophage inflammation model were employed. Analyses included the assessment of intestinal injury, motility, inflammatory cytokines, macrophage infiltration, and polarization. RNA sequencing implicated the JAK2/STAT3 pathway, which was validated through immunohistochemistry and Western blot. Subsequent STAT3 knockout and overexpression experiments in macrophages further elucidated the underlying mechanism.
Results: ω-3 PUFAs alleviated intestinal damage, restored motility, and reduced local pro-inflammatory cytokine levels in POI mice, along with reduced macrophage infiltration. In vitro, ω-3 PUFAs suppressed M1 polarization. RNA-seq implicated the JAK/STAT pathway, and further experiments confirmed that ω-3 PUFAs markedly suppressed the phosphorylation of JAK2, STAT3, and p65 in both macrophages and ileal tissue. Genetic manipulation of STAT3 established that ω-3 PUFAs attenuate inflammation primarily through modulating macrophage polarization.
Conclusion: ω-3 PUFAs alleviate POI by inhibiting the JAK2/STAT3 signaling pathway in macrophages, thereby suppressing their polarization toward the pro-inflammatory M1 phenotype and reducing local intestinal inflammation. These findings indicate that ω-3 PUFAs may be a promising prophylactic or therapeutic agent for POI.
Keywords: omega-3 polyunsaturated fatty acids, postoperative ileus, macrophage polarization, inflammatory cytokines, JAK2/STAT3 signaling pathway