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已发表论文
基于转录组测序和生物信息学分析探讨电针对肠易激综合征大鼠模型前扣带皮层表达谱的影响
Authors Guo Y, Wang D , Chen M, Wei X, Yang Z, Wang Z, Zhang H
Received 14 October 2025
Accepted for publication 13 January 2026
Published 2 February 2026 Volume 2026:19 574208
DOI https://doi.org/10.2147/JPR.S574208
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Houman Danesh
Yixuan Guo,1 Dan Wang,2 Mengyuan Chen,2 Xinyu Wei,2 Zihan Yang,2 Zhen Wang,3 Haiyan Zhang3
1College of Acupuncture-Moxibustion and Tuina, Shandong University of Traditional Chinese Medicine, Jinan, 250355, People’s Republic of China; 2College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250355, People’s Republic of China; 3Research Institute of Acupuncture and Moxibustion, Shandong University of Traditional Chinese Medicine, Jinan, 250355, People’s Republic of China
Correspondence: Haiyan Zhang, Shandong University of Traditional Chinese Medicine, Jinan, 250355, People’s Republic of China, Email zhy2013@sdutcm.edu.cn
Purpose: Given that electroacupuncture (EA) is a promising yet mechanistically unclear intervention for IBS, and considering the anterior cingulate cortex (ACC)’s crucial role in visceral pain and emotion within the gut-brain axis, this study aims to investigate the transcriptomic alterations in the ACC of a rat IBS model and to evaluate the potential therapeutic effects of EA administered at the acupoints Tianshu (ST25) and Zusanli (ST36).
Methods: Rats subjected to water avoidance stress (WAS) were evaluated for IBS symptoms, including increased anxiety-like behavior, abnormal abdominal muscle activity, and elevated abdominal withdrawal reflex (AWR) scores—a validated measure of visceral hypersensitivity. The effect of EA at ST25 and ST36 was assessed on these symptoms, and the transcriptomic profile of the ACC was analyzed after WAS and EA treatment using RNA sequencing and subsequent validation by real-time PCR.
Results: The stress model induced significant IBS-like symptoms, including visceral hypersensitivity and anxiety-like behavior. EA at ST25 and ST36 significantly ameliorated these behavioral and physiological deficits. Notably, transcriptomic profiling of the ACC linked this behavioral improvement to central modulation, revealing dysregulation of 3 key genes involved in many pathways. RT-PCR validated the expression changes in 3 selected candidate genes, further supporting their role in EA’s therapeutic effects.
Conclusion: EA alleviates IBS symptoms in rats, potentially through transcriptomic reprogramming in the ACC, which provides novel mechanistic insight into how peripheral acupuncture may exert central actions to treat IBS.
Keywords: anterior cingulate cortex, irritable bowel syndrome, transcriptome sequencing analysis, electroacupuncture
1College of Acupuncture-Moxibustion and Tuina, Shandong University of Traditional Chinese Medicine, Jinan, 250355, People’s Republic of China; 2College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250355, People’s Republic of China; 3Research Institute of Acupuncture and Moxibustion, Shandong University of Traditional Chinese Medicine, Jinan, 250355, People’s Republic of China
Correspondence: Haiyan Zhang, Shandong University of Traditional Chinese Medicine, Jinan, 250355, People’s Republic of China, Email zhy2013@sdutcm.edu.cn
Purpose: Given that electroacupuncture (EA) is a promising yet mechanistically unclear intervention for IBS, and considering the anterior cingulate cortex (ACC)’s crucial role in visceral pain and emotion within the gut-brain axis, this study aims to investigate the transcriptomic alterations in the ACC of a rat IBS model and to evaluate the potential therapeutic effects of EA administered at the acupoints Tianshu (ST25) and Zusanli (ST36).
Methods: Rats subjected to water avoidance stress (WAS) were evaluated for IBS symptoms, including increased anxiety-like behavior, abnormal abdominal muscle activity, and elevated abdominal withdrawal reflex (AWR) scores—a validated measure of visceral hypersensitivity. The effect of EA at ST25 and ST36 was assessed on these symptoms, and the transcriptomic profile of the ACC was analyzed after WAS and EA treatment using RNA sequencing and subsequent validation by real-time PCR.
Results: The stress model induced significant IBS-like symptoms, including visceral hypersensitivity and anxiety-like behavior. EA at ST25 and ST36 significantly ameliorated these behavioral and physiological deficits. Notably, transcriptomic profiling of the ACC linked this behavioral improvement to central modulation, revealing dysregulation of 3 key genes involved in many pathways. RT-PCR validated the expression changes in 3 selected candidate genes, further supporting their role in EA’s therapeutic effects.
Conclusion: EA alleviates IBS symptoms in rats, potentially through transcriptomic reprogramming in the ACC, which provides novel mechanistic insight into how peripheral acupuncture may exert central actions to treat IBS.
Keywords: anterior cingulate cortex, irritable bowel syndrome, transcriptome sequencing analysis, electroacupuncture