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已发表论文
蛋白质组芯片引导的全局视角：淫羊藿苷的多重药理靶点
Authors Gong G, Tang X, Yuan G, Yang X, Wang G, Zhang F
Received 14 November 2025
Accepted for publication 24 January 2026
Published 3 February 2026 Volume 2026:20 581728
DOI https://doi.org/10.2147/DDDT.S581728
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Anastasios Lymperopoulos
Guichun Gong,1,* Xianjin Tang,1,* Guanghun Yuan,1 Xinxing Yang,2 Guoqing Wang,1 Feng Zhang1,2
1Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education and Key Laboratory of Basic Pharmacology of Guizhou Province and Laboratory Animal Center, Zunyi Medical University, Zunyi, Guizhou, 563000, People’s Republic of China; 2State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, Guizhou, 561113, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Feng Zhang, Email zhangfengzmc@163.com
Background: Icariin (ICA) is a major bioactive compound extracted from the traditional Chinese medicinal herb Epimedium, demonstrated a broad spectrum of pharmacological properties. However, a comprehensive and up-to-date study exclusively focusing on ICA direct binding proteins has not yet been conducted.
Methods: HuProt™ 20K human proteome microarray, the highest capacity human protein microarray, is suitable for the applications of small molecule targeting proteins. Here, we constructed an atlas of the ICA-binding proteins directly via proteome microarray.
Results: We showed that a total of 246 proteins which directly interacted with ICA. Subsequent PPI network analysis organized these proteins into 4 functionally distinct clusters, revealing that ICA interacts with proteins central to fundamental cellular processes, including protein folding chaperone complexes, the ubiquitin-proteasome system, apoptotic and PI3K-Akt signaling pathways, and nucleotide metabolism.
Conclusion: This study constructed an atlas of the ICA-binding proteins directly via microarray, which spans protein folding, ubiquitin-proteasome, apoptosis and nucleotide metabolism, providing a theoretical basis for ICA treatment of diseases caused by dysregulation of these core pathways.
Keywords: icariin, proteome microarray, pharmacological targets, protein networks, molecular mechanism
1Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education and Key Laboratory of Basic Pharmacology of Guizhou Province and Laboratory Animal Center, Zunyi Medical University, Zunyi, Guizhou, 563000, People’s Republic of China; 2State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Guizhou Medical University, Guiyang, Guizhou, 561113, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Feng Zhang, Email zhangfengzmc@163.com
Background: Icariin (ICA) is a major bioactive compound extracted from the traditional Chinese medicinal herb Epimedium, demonstrated a broad spectrum of pharmacological properties. However, a comprehensive and up-to-date study exclusively focusing on ICA direct binding proteins has not yet been conducted.
Methods: HuProt™ 20K human proteome microarray, the highest capacity human protein microarray, is suitable for the applications of small molecule targeting proteins. Here, we constructed an atlas of the ICA-binding proteins directly via proteome microarray.
Results: We showed that a total of 246 proteins which directly interacted with ICA. Subsequent PPI network analysis organized these proteins into 4 functionally distinct clusters, revealing that ICA interacts with proteins central to fundamental cellular processes, including protein folding chaperone complexes, the ubiquitin-proteasome system, apoptotic and PI3K-Akt signaling pathways, and nucleotide metabolism.
Conclusion: This study constructed an atlas of the ICA-binding proteins directly via microarray, which spans protein folding, ubiquitin-proteasome, apoptosis and nucleotide metabolism, providing a theoretical basis for ICA treatment of diseases caused by dysregulation of these core pathways.
Keywords: icariin, proteome microarray, pharmacological targets, protein networks, molecular mechanism