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已发表论文
肺部感染病原体检测中宏基因组二代测序与传统培养方法的比较评估
Authors Chen W, Liu R, Qi Q, Xu L, Sun G
Received 20 June 2025
Accepted for publication 20 December 2025
Published 30 December 2025 Volume 2025:18 Pages 6991—6998
DOI https://doi.org/10.2147/IDR.S547686
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Alberto Ospina Stella
Weijun Chen,1,* Ruijie Liu,1,* Qiyong Qi,1 Lingen Xu,2 Guiqin Sun3
1Laboratory Department, Xinchang Hospital of Traditional Chinese Medicine, Shaoxing, Zhejiang, People’s Republic of China; 2Intensive Care Unit, Xinchang Hospital of Traditional Chinese Medicine, Shaoxing, Zhejiang, People’s Republic of China; 3School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Guiqin Sun, School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou, Zhejiang, People’s Republic of China, 310053, Tel +86 13868118601, Fax +86 0571-86633307, Email sunguiqin2001@163.com Lingen Xu, Intensive Care Unit, Xinchang Hospital of Traditional Chinese Medicine, No. 188, Shijiu Feng Road, Qixing Street, Shaoxing, Zhejiang, People’s Republic of China, 312500, Tel +86 13857514222, Fax +86 0575-86502818, Email xlger@163.com
Purpose: This study aimed to evaluate the diagnostic accuracy and clinical applicability of metagenomic next-generation sequencing (mNGS) in pulmonary infections by comparing it with traditional culture methods in a Traditional Chinese Medicine (TCM) hospital setting.
Methods: This retrospective cohort study enrolled 67 consecutively admitted patients with radiologically and clinically confirmed pulmonary infections from the Department of Respiratory Infectious Diseases at Xinchang Hospital of Traditional Chinese Medicine between December 2022 and September 2024. Clinical specimens included blood, bronchoalveolar lavage fluid (BALF), sputum, hydrothorax and cerebrospinal fluid (CSF). mNGS and conventional culture were performed to compare detection rates and microbial community profiles.
Results: Among 67 cases, mNGS identified pathogens in 89.55% (60/67), compared to 20.90% (14/67) by traditional culture. Of 14 dual-positive cases, only 1 (1/14, 7.14%) showed complete concordance, while most exhibited discordance or partial genus-level overlap. mNGS further detected viral co-infections in 44.78% (30/67) and identified fastidious/non-culturable pathogens such as enterovirus, human herpesvirus type 1, and Mycobacterium tuberculosis. Patients with chronic diseases were more susceptible to EB virus infections.
Conclusion: mNGS significantly enhances pathogen detection in pulmonary infections, supports targeted antimicrobial therapy, and holds potential for contributing to clinical outcomes and reducing antibiotic resistance.
Keywords: mNGS, pulmonary infection, pathogens, antibiotic resistance, detection
1Laboratory Department, Xinchang Hospital of Traditional Chinese Medicine, Shaoxing, Zhejiang, People’s Republic of China; 2Intensive Care Unit, Xinchang Hospital of Traditional Chinese Medicine, Shaoxing, Zhejiang, People’s Republic of China; 3School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Guiqin Sun, School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang District, Hangzhou, Zhejiang, People’s Republic of China, 310053, Tel +86 13868118601, Fax +86 0571-86633307, Email sunguiqin2001@163.com Lingen Xu, Intensive Care Unit, Xinchang Hospital of Traditional Chinese Medicine, No. 188, Shijiu Feng Road, Qixing Street, Shaoxing, Zhejiang, People’s Republic of China, 312500, Tel +86 13857514222, Fax +86 0575-86502818, Email xlger@163.com
Purpose: This study aimed to evaluate the diagnostic accuracy and clinical applicability of metagenomic next-generation sequencing (mNGS) in pulmonary infections by comparing it with traditional culture methods in a Traditional Chinese Medicine (TCM) hospital setting.
Methods: This retrospective cohort study enrolled 67 consecutively admitted patients with radiologically and clinically confirmed pulmonary infections from the Department of Respiratory Infectious Diseases at Xinchang Hospital of Traditional Chinese Medicine between December 2022 and September 2024. Clinical specimens included blood, bronchoalveolar lavage fluid (BALF), sputum, hydrothorax and cerebrospinal fluid (CSF). mNGS and conventional culture were performed to compare detection rates and microbial community profiles.
Results: Among 67 cases, mNGS identified pathogens in 89.55% (60/67), compared to 20.90% (14/67) by traditional culture. Of 14 dual-positive cases, only 1 (1/14, 7.14%) showed complete concordance, while most exhibited discordance or partial genus-level overlap. mNGS further detected viral co-infections in 44.78% (30/67) and identified fastidious/non-culturable pathogens such as enterovirus, human herpesvirus type 1, and Mycobacterium tuberculosis. Patients with chronic diseases were more susceptible to EB virus infections.
Conclusion: mNGS significantly enhances pathogen detection in pulmonary infections, supports targeted antimicrobial therapy, and holds potential for contributing to clinical outcomes and reducing antibiotic resistance.
Keywords: mNGS, pulmonary infection, pathogens, antibiotic resistance, detection