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已发表论文
四君子抗病毒合剂通过下调 TNF/IL-17 信号通路改善脂多糖诱导的急性肺损伤：基于转录组学、网络药理学和实验验证的综合研究
Authors Wang Y, Zhou H, Wang B, Liu Y, Long K, Zhang H
Received 27 August 2025
Accepted for publication 21 November 2025
Published 30 December 2025 Volume 2025:18 Pages 18267—18290
DOI https://doi.org/10.2147/JIR.S562152
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Tara Strutt
Yuan Wang,1 Huiying Zhou,1 Bo Wang,2 Yuxi Liu,1 Kaihua Long,1 Hong Zhang1
1Institute of Chinese Materia Medica, Shaanxi Academy of Traditional Chinese Medicine (Shaanxi Provincial Hospital of Traditional Chinese Medicine), Xi’an, People’s Republic of China; 2College of Life Sciences, Northwest University, Xi’an, People’s Republic of China
Correspondence: Kaihua Long, Shaanxi Academy of Traditional Chinese Medicine (Shaanxi Provincial Hospital of Traditional Chinese Medicine), No. 4 Xihuamen, Lianhu District, Xi’an, 710003, People’s Republic of China, Email longkaihua@126.com Hong Zhang, Shaanxi Academy of Traditional Chinese Medicine (Shaanxi Provincial Hospital of Traditional Chinese Medicine), No. 4 Xihuamen, Lianhu District, Xi’an, 710003, People’s Republic of China, Email zhanghong919919@163.com
Background: Acute lung injury (ALI), as a respiratory condition triggered by various internal and external factors, is characterized clinically by high rates of incidence and mortality. In the current context where respiratory system diseases are on the rise, the incidence of ALI has been increasing annually. Previous research has established the efficacy of traditional Chinese medicine (TCM) in inhibiting ALI. Siji Kangbingdu Mixture (SJM), has demonstrated a clear clinical therapeutic effect in managing respiratory infections. Nevertheless, its mechanism of action in treating ALI is still lacking.
Purpose: To clarify the mechanism by which SJM exerts its therapeutic effects in the treatment of ALI.
Materials and Methods: In our study, we initially elucidated the chemical constituents of SJM by the UHPLC-Q-Orbitrap HRMS (LC-MS), and established an ALI model. Next, we evaluated how SJM inhibited ALI by measuring the weight curve of mice, the wet-to-dry lung weight ratio (W/D), the HE lung tissue pathological change score, along with the inflammatory factor expression. Then, by employing transcriptomics and network pharmacology approaches, the mechanism of SJM acting on ALI were predicted and analyzed. Finally, the expression and regulatory interactions of the differentially expressed target proteins in the key pathways were verified by qRT-PCR analysis, Western blotting (WB), immunofluorescence and immunohistochemistry.
Results: The LC-MS analysis identified 148 active ingredients. In vitro experiments indicated that SJM could significantly reduce the W/D index, pulmonary tissue pathological score, and the inflammatory factor levels. The findings from transcriptomics and network pharmacology suggested that SJM may alleviate ALI as a TNF and IL-17 signaling pathway. Further, through WB, immunofluorescence and immunohistochemistry, we noted significant downregulations in the pulmonary tissue expression of keys proteins in the SJM-H group.
Conclusion: SJM has the potential to inhibit the progression of ALI, and its mechanism of action may involve the TNF and IL-17 signaling downregulation.
Keywords: Siji kangbingdu mixture, acute lung injury, network pharmacology, transcriptomics, TNF signaling pathway, IL-17 signaling pathway
1Institute of Chinese Materia Medica, Shaanxi Academy of Traditional Chinese Medicine (Shaanxi Provincial Hospital of Traditional Chinese Medicine), Xi’an, People’s Republic of China; 2College of Life Sciences, Northwest University, Xi’an, People’s Republic of China
Correspondence: Kaihua Long, Shaanxi Academy of Traditional Chinese Medicine (Shaanxi Provincial Hospital of Traditional Chinese Medicine), No. 4 Xihuamen, Lianhu District, Xi’an, 710003, People’s Republic of China, Email longkaihua@126.com Hong Zhang, Shaanxi Academy of Traditional Chinese Medicine (Shaanxi Provincial Hospital of Traditional Chinese Medicine), No. 4 Xihuamen, Lianhu District, Xi’an, 710003, People’s Republic of China, Email zhanghong919919@163.com
Background: Acute lung injury (ALI), as a respiratory condition triggered by various internal and external factors, is characterized clinically by high rates of incidence and mortality. In the current context where respiratory system diseases are on the rise, the incidence of ALI has been increasing annually. Previous research has established the efficacy of traditional Chinese medicine (TCM) in inhibiting ALI. Siji Kangbingdu Mixture (SJM), has demonstrated a clear clinical therapeutic effect in managing respiratory infections. Nevertheless, its mechanism of action in treating ALI is still lacking.
Purpose: To clarify the mechanism by which SJM exerts its therapeutic effects in the treatment of ALI.
Materials and Methods: In our study, we initially elucidated the chemical constituents of SJM by the UHPLC-Q-Orbitrap HRMS (LC-MS), and established an ALI model. Next, we evaluated how SJM inhibited ALI by measuring the weight curve of mice, the wet-to-dry lung weight ratio (W/D), the HE lung tissue pathological change score, along with the inflammatory factor expression. Then, by employing transcriptomics and network pharmacology approaches, the mechanism of SJM acting on ALI were predicted and analyzed. Finally, the expression and regulatory interactions of the differentially expressed target proteins in the key pathways were verified by qRT-PCR analysis, Western blotting (WB), immunofluorescence and immunohistochemistry.
Results: The LC-MS analysis identified 148 active ingredients. In vitro experiments indicated that SJM could significantly reduce the W/D index, pulmonary tissue pathological score, and the inflammatory factor levels. The findings from transcriptomics and network pharmacology suggested that SJM may alleviate ALI as a TNF and IL-17 signaling pathway. Further, through WB, immunofluorescence and immunohistochemistry, we noted significant downregulations in the pulmonary tissue expression of keys proteins in the SJM-H group.
Conclusion: SJM has the potential to inhibit the progression of ALI, and its mechanism of action may involve the TNF and IL-17 signaling downregulation.
Keywords: Siji kangbingdu mixture, acute lung injury, network pharmacology, transcriptomics, TNF signaling pathway, IL-17 signaling pathway