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已发表论文
齐墩果酸通过过氧化物酶体增殖物激活受体γ相关机制对葡聚糖硫酸钠所致小鼠溃疡性结肠炎的预防作用
Authors Tian G, Dong F , Fu A , Lai S , Yu J , Ni J, Ding Q, Jin Y
Received 11 September 2025
Accepted for publication 13 December 2025
Published 25 December 2025 Volume 2025:19 Pages 11641—11657
DOI https://doi.org/10.2147/DDDT.S560422
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Professor Anastasios Lymperopoulos
Guojiang Tian,1,* Fan Dong,2,* Ai Fu,3,* Shanglei Lai,4 Jundi Yu,2 Jingjing Ni,5 Qinchao Ding,2 Yueping Jin5
1Department of Anus and Colorectal Surgery, Shaoxing People’s Hospital, Shaoxing, Zhejiang, People’s Republic of China; 2School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China; 3Institute of Hepatology and Epidemiology, Affiliated Hangzhou Xixi Hospital, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China; 4Department of Medical Research Center, Shaoxing People’s Hospital, Shaoxing, Zhejiang, People’s Republic of China; 5Department of Gastroenterology, Shaoxing People’s Hospital, Shaoxing, Zhejiang, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Qinchao Ding, School of Public Health, Zhejiang Chinese Medical University, No. 548, Binwen Road, Binjiang District, Hangzhou, Zhejiang, 310053, People’s Republic of China, Email dingqinchao@zcmu.edu.cn Yueping Jin, Department of Gastroenterology, Shaoxing People’s Hospital, No. 568, Zhongxing North Road, Yuecheng District, Shaoxing, Zhejiang, 312000, People’s Republic of China, Email jinyueping0424@163.com
Purpose: Oleanolic acid (OA), a naturally occurring pentacyclic triterpenoid, has been reported to possess anti-inflammatory and barrier-protective properties. However, its molecular mechanism in the context of ulcerative colitis (UC) remains unclear.
Methods: In this study, we evaluated the therapeutic potential of OA (25 and 50 mg/kg/day) in a dextran sulfate sodium (DSS)-induced acute colitis model in C57BL/6 mice.
Results: Oral administration of OA significantly alleviated clinical symptoms of colitis, including weight loss, colon shortening, increased disease activity index (DAI), and histopathological injury. Additionally, OA demonstrated potent anti-inflammatory effects by suppressing the production of pro-inflammatory cytokines and promoting anti-inflammatory cytokines. It also restored redox homeostasis by enhancing antioxidant defenses and reducing lipid peroxidation. OA also restored intestinal barrier integrity, as evidenced by increased tight junction protein expression and decreased intestinal permeability. Mechanistically, OA was found to inhibit the activation of the NF-κB signaling pathway, as evidenced by reduced phosphorylation of IKKα/β and p65, and decreased degradation of IκBα. A key finding was that OA restored the expression of PPARγ, a known negative regulator of NF-κB. OA promoted the accumulation of peroxisome proliferator-activated receptor gamma (PPARγ), a nuclear receptor essential for maintaining intestinal epithelial homeostasis and anti-inflammatory signaling. OA inhibited the ubiquitin-mediated proteasomal degradation of PPARγ, thereby stabilizing its protein levels in colonic tissue. Pharmacological inhibition of PPARγ with GW9662 abolished the protective effects of OA on intestinal inflammation and epithelial barrier function, confirming that PPARγ activation is required for OA-mediated protection.
Conclusion: These findings identify a previously unrecognized mechanism whereby OA protects against colitis through post-translational stabilization of PPARγ. Our study not only highlights the therapeutic value of OA as a potential intervention for UC but also provides mechanistic insight into the regulation of nuclear receptor homeostasis during intestinal inflammation.
Keywords: oleanolic acid, ulcerative colitis, inflammation, PPARγ, intestinal barrier function
1Department of Anus and Colorectal Surgery, Shaoxing People’s Hospital, Shaoxing, Zhejiang, People’s Republic of China; 2School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China; 3Institute of Hepatology and Epidemiology, Affiliated Hangzhou Xixi Hospital, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China; 4Department of Medical Research Center, Shaoxing People’s Hospital, Shaoxing, Zhejiang, People’s Republic of China; 5Department of Gastroenterology, Shaoxing People’s Hospital, Shaoxing, Zhejiang, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Qinchao Ding, School of Public Health, Zhejiang Chinese Medical University, No. 548, Binwen Road, Binjiang District, Hangzhou, Zhejiang, 310053, People’s Republic of China, Email dingqinchao@zcmu.edu.cn Yueping Jin, Department of Gastroenterology, Shaoxing People’s Hospital, No. 568, Zhongxing North Road, Yuecheng District, Shaoxing, Zhejiang, 312000, People’s Republic of China, Email jinyueping0424@163.com
Purpose: Oleanolic acid (OA), a naturally occurring pentacyclic triterpenoid, has been reported to possess anti-inflammatory and barrier-protective properties. However, its molecular mechanism in the context of ulcerative colitis (UC) remains unclear.
Methods: In this study, we evaluated the therapeutic potential of OA (25 and 50 mg/kg/day) in a dextran sulfate sodium (DSS)-induced acute colitis model in C57BL/6 mice.
Results: Oral administration of OA significantly alleviated clinical symptoms of colitis, including weight loss, colon shortening, increased disease activity index (DAI), and histopathological injury. Additionally, OA demonstrated potent anti-inflammatory effects by suppressing the production of pro-inflammatory cytokines and promoting anti-inflammatory cytokines. It also restored redox homeostasis by enhancing antioxidant defenses and reducing lipid peroxidation. OA also restored intestinal barrier integrity, as evidenced by increased tight junction protein expression and decreased intestinal permeability. Mechanistically, OA was found to inhibit the activation of the NF-κB signaling pathway, as evidenced by reduced phosphorylation of IKKα/β and p65, and decreased degradation of IκBα. A key finding was that OA restored the expression of PPARγ, a known negative regulator of NF-κB. OA promoted the accumulation of peroxisome proliferator-activated receptor gamma (PPARγ), a nuclear receptor essential for maintaining intestinal epithelial homeostasis and anti-inflammatory signaling. OA inhibited the ubiquitin-mediated proteasomal degradation of PPARγ, thereby stabilizing its protein levels in colonic tissue. Pharmacological inhibition of PPARγ with GW9662 abolished the protective effects of OA on intestinal inflammation and epithelial barrier function, confirming that PPARγ activation is required for OA-mediated protection.
Conclusion: These findings identify a previously unrecognized mechanism whereby OA protects against colitis through post-translational stabilization of PPARγ. Our study not only highlights the therapeutic value of OA as a potential intervention for UC but also provides mechanistic insight into the regulation of nuclear receptor homeostasis during intestinal inflammation.
Keywords: oleanolic acid, ulcerative colitis, inflammation, PPARγ, intestinal barrier function