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已发表论文
银花利湿汤通过调节辅助性 T 细胞平衡和修复上皮屏障缓解特应性皮炎
Authors Wu J , Tai Z , Zhu C, Li L, Liu J, Ma T, Yin B, Zhou H, Zhu Q, Chen Z
Received 8 May 2025
Accepted for publication 23 August 2025
Published 21 October 2025 Volume 2025:18 Pages 14569—14587
DOI https://doi.org/10.2147/JIR.S531656
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Anish R. Maskey
Junchao Wu,1,2,* Zongguang Tai,1,2,* Congcong Zhu,1,2 Lisha Li,1,2 Jun Liu,1,2 Tianyou Ma,1,2 Bei Yin,1,2 Hanxue Zhou,1,2 Quangang Zhu,1,2 Zhongjian Chen1,2
1Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 200443, People’s Republic of China; 2Shanghai Engineering Research Center for Topical Chinese Medicine, Shanghai, 200443, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Zhongjian Chen, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, 1278 Baode Road, Shanghai, 200443, People’s Republic of China, Email aajian818@163.com Quangang Zhu, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, 1278 Baode Road, Shanghai, 200443, People’s Republic of China, Email qgzhu@126.com
Purpose: Yin-hua Li-shi Decoction (YLD), a traditional Chinese medicine formulation, has been employed as a complementary therapy for atopic dermatitis (AD). Nevertheless, its precise therapeutic mechanisms remained unexplored.
Methods: YLD components were identified by LC-MS, and quality control was performed using HPLC. In vitro, flow cytometry was used to assess YLD toxicity and its effect on inhibiting pro-inflammatory macrophages. In vivo, AD mice model was induced by daily topical MC903 application on mice ears for 15 days. Mice received oral YLD (1.50, 3.00, or 6.00 g/mL) once daily for 14 days. Disease progression was tracked by measuring skin thickness in mice. ELISA, western blot, and immunohistochemistry were used to analyze YLD ability to regulate inflammatory factors and restore skin barrier proteins. Flow cytometry was additionally used to investigate YLD modulatory effects on type I, II, and III adaptive immune responses.
Results: YLD could inhibit the differentiation of macrophages into M1 phenotype in vitro. In the AD-like mice, YLD ameliorated epidermal hyperkeratosis and skin lesions, decreased the severity scoring of AD, and suppressed the inflammation of the skin in a dose-dependent manner. Moreover, YLD downregulated pro-inflammatory factors such as IL-4/13, TNF-α, TSLP, and IgE antibodies, restored the expression of barrier proteins in the skin, decreased the infiltration of CD4+ T cells in AD skin, and reestablished the balance of Th1/ Th2/ Th17 cells.
Conclusion: YLD alleviated AD by regulating the adaptive immune response through modulation of T cell differentiation and cytokine production, and by restoring skin barrier function. The study revealed the therapeutic mechanism of YLD and provided experimental evidence supporting its application in AD treatment.
Keywords: atopic dermatitis, Yin-hua Li-shi decoction, inflammatory factors, helper T cells, skin barrier function
1Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 200443, People’s Republic of China; 2Shanghai Engineering Research Center for Topical Chinese Medicine, Shanghai, 200443, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Zhongjian Chen, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, 1278 Baode Road, Shanghai, 200443, People’s Republic of China, Email aajian818@163.com Quangang Zhu, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, 1278 Baode Road, Shanghai, 200443, People’s Republic of China, Email qgzhu@126.com
Purpose: Yin-hua Li-shi Decoction (YLD), a traditional Chinese medicine formulation, has been employed as a complementary therapy for atopic dermatitis (AD). Nevertheless, its precise therapeutic mechanisms remained unexplored.
Methods: YLD components were identified by LC-MS, and quality control was performed using HPLC. In vitro, flow cytometry was used to assess YLD toxicity and its effect on inhibiting pro-inflammatory macrophages. In vivo, AD mice model was induced by daily topical MC903 application on mice ears for 15 days. Mice received oral YLD (1.50, 3.00, or 6.00 g/mL) once daily for 14 days. Disease progression was tracked by measuring skin thickness in mice. ELISA, western blot, and immunohistochemistry were used to analyze YLD ability to regulate inflammatory factors and restore skin barrier proteins. Flow cytometry was additionally used to investigate YLD modulatory effects on type I, II, and III adaptive immune responses.
Results: YLD could inhibit the differentiation of macrophages into M1 phenotype in vitro. In the AD-like mice, YLD ameliorated epidermal hyperkeratosis and skin lesions, decreased the severity scoring of AD, and suppressed the inflammation of the skin in a dose-dependent manner. Moreover, YLD downregulated pro-inflammatory factors such as IL-4/13, TNF-α, TSLP, and IgE antibodies, restored the expression of barrier proteins in the skin, decreased the infiltration of CD4+ T cells in AD skin, and reestablished the balance of Th1/ Th2/ Th17 cells.
Conclusion: YLD alleviated AD by regulating the adaptive immune response through modulation of T cell differentiation and cytokine production, and by restoring skin barrier function. The study revealed the therapeutic mechanism of YLD and provided experimental evidence supporting its application in AD treatment.
Keywords: atopic dermatitis, Yin-hua Li-shi decoction, inflammatory factors, helper T cells, skin barrier function