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已发表论文
慢性阻塞性肺疾病患者红细胞的蛋白质组学分析揭示蛋白酶体激活与细胞形态和功能异常之间的关联
Authors Wang XL, Zhong K , Li R, Huang LH, Chen GJ, Chai JW, Chen X
Received 29 March 2025
Accepted for publication 18 September 2025
Published 22 September 2025 Volume 2025:20 Pages 3279—3294
DOI https://doi.org/10.2147/COPD.S531220
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 4
Editor who approved publication: Professor Richard Russell
Xi-Long Wang,1,* Kai Zhong,1,* Rui Li,1,* Lin-Hui Huang,1,2 Guan-Jin Chen,1 Jin-Wei Chai,1 Xin Chen1
1Department of Pulmonary and Critical Care Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, People’s Republic of China; 2Department of Pulmonary and Critical Care Medicine, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou, Hainan, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Jin-Wei Chai, Department of Pulmonary and Critical Care Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People’s Republic of China, Tel +86 20 62782296, Fax +86 20 61643010, Email vivian20@i.smu.edu.cn Xin Chen, Department of Pulmonary and Critical Care Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People’s Republic of China, Tel +86 20 62782296, Fax +86 20 61643010, Email hxkd006@smu.edu.cn
Introduction: Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow obstruction and persistent respiratory symptoms. Molecular and cellular changes identified in red blood cells (RBCs) of COPD patients may contribute to the pathophysiology of COPD, impacting oxygen transport and systemic inflammation.
Methods: We performed a comparative proteomic analysis on RBCs from 15 male COPD patients and 15 age- and sex-matched control subjects. For the proteomic analysis, individual samples were randomly pooled into 3 biological replicates per group (n = 3). Total RBC proteins were analyzed using tandem mass tag (TMT) labeling followed by LC-MS/MS. Differentially abundant proteins (DAPs) were identified and subjected to Gene Ontology (GO), KEGG pathway, and protein-protein interaction (PPI) network analyses.
Results: We identified 160 DAPs (70 up-regulated, 90 down-regulated) in the RBCs of COPD patients. GO analysis revealed enrichment in processes related to protein stability regulation and immune response. KEGG pathway analysis showed that up-regulated proteins were most significantly enriched in the proteasome pathway, while down-regulated proteins were enriched in complement and coagulation cascades. Notably, a PPI network analysis highlighted a core complex of 10 up-regulated proteins that are all components of the proteasome regulatory particle.
Conclusion: This study provides the in-depth RBC protein profile in COPD, identifying proteasome activation as a key molecular signature. These findings reveal novel biomarkers linked to RBC dysfunction that may contribute to the systemic pathology of COPD and offer potential new therapeutic targets.
Keywords: COPD, red blood cell, proteasome, proteomics
1Department of Pulmonary and Critical Care Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, People’s Republic of China; 2Department of Pulmonary and Critical Care Medicine, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou, Hainan, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Jin-Wei Chai, Department of Pulmonary and Critical Care Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People’s Republic of China, Tel +86 20 62782296, Fax +86 20 61643010, Email vivian20@i.smu.edu.cn Xin Chen, Department of Pulmonary and Critical Care Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People’s Republic of China, Tel +86 20 62782296, Fax +86 20 61643010, Email hxkd006@smu.edu.cn
Introduction: Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow obstruction and persistent respiratory symptoms. Molecular and cellular changes identified in red blood cells (RBCs) of COPD patients may contribute to the pathophysiology of COPD, impacting oxygen transport and systemic inflammation.
Methods: We performed a comparative proteomic analysis on RBCs from 15 male COPD patients and 15 age- and sex-matched control subjects. For the proteomic analysis, individual samples were randomly pooled into 3 biological replicates per group (n = 3). Total RBC proteins were analyzed using tandem mass tag (TMT) labeling followed by LC-MS/MS. Differentially abundant proteins (DAPs) were identified and subjected to Gene Ontology (GO), KEGG pathway, and protein-protein interaction (PPI) network analyses.
Results: We identified 160 DAPs (70 up-regulated, 90 down-regulated) in the RBCs of COPD patients. GO analysis revealed enrichment in processes related to protein stability regulation and immune response. KEGG pathway analysis showed that up-regulated proteins were most significantly enriched in the proteasome pathway, while down-regulated proteins were enriched in complement and coagulation cascades. Notably, a PPI network analysis highlighted a core complex of 10 up-regulated proteins that are all components of the proteasome regulatory particle.
Conclusion: This study provides the in-depth RBC protein profile in COPD, identifying proteasome activation as a key molecular signature. These findings reveal novel biomarkers linked to RBC dysfunction that may contribute to the systemic pathology of COPD and offer potential new therapeutic targets.
Keywords: COPD, red blood cell, proteasome, proteomics