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量子点侧向免疫层析结合图像分析技术半定量检测尘螨特异性 IgE
Authors Zhao Y, Zhang Q, Meng Q, Wu F, Zhang L, Tang Y, Guan Y, An L
Received 12 February 2017
Accepted for publication 23 May 2017
Published 4 July 2017 Volume 2017:12 Pages 4805—4812
DOI https://doi.org/10.2147/IJN.S134539
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Lakshmi Kiran Chelluri
Peer reviewer comments 2
Editor who approved publication: Dr Linlin Sun
摘要：由于空气污染加重导致鼻炎和哮喘的流行，临床相关性优良的半定量、快速检测室尘螨特异性 IgE（sIgE）应用前面广阔。然而，传统的 IgE 检测方法通常耗时长、步骤复杂、需要特殊的仪器。本研究为克服目前 sIgE 检测方法的缺陷，我们开发了一种基于量子点纳米球的侧向免疫层析和图像分析技术来检测 sIgE 的方法。该检测方法可以在 0.2-10U/mL 范围内半定量检测 IgE。进一步，通过检测 56 例阳性病人和 40 例健康对照组的血清，检测结果与临床症状具有很好的相关性。本检测方法具有简单、灵活、使用方便、适于家庭检测等特点。
Keywords: lateral flow immunoassay, quantum
dots, house dust mite, IgE
