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已发表论文
惠阳生肌膏促进糖尿病慢性伤口的淋巴管生成和伤口愈合：来自蛋白质组学及体内体外分析的综合见解
Authors Yu F, Lin L, Tang X, He X, Xu X
Received 29 May 2025
Accepted for publication 10 September 2025
Published 16 September 2025 Volume 2025:18 Pages 12883—12908
DOI https://doi.org/10.2147/JIR.S534105
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Qing Lin
Fangning Yu,* Li Lin,* Xiao Tang,* Xiujuan He, Xuying Xu
Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Xuying Xu, Beijing Hospital of Traditional Chinese Medicine, No. 23, Museum Back Street, Dongcheng District, Beijing, 10010, People’s Republic of China, Tel +86- 010-87906642, Email xxying7341@126.com
Purpose: To investigate the mechanism by which HYSJ unguent promotes lymphangiogenesis and improves the healing of diabetic chronic wounds (DCWs).
Methods: The main components of HYSJ were identified by mass spectrometry. A mouse model with chronic skin ulcers was established. The ultrastructure and lymphatic drainage of lymphatic endothelial cells in wounds were examined. Lymphatic markers in wound tissues were detected, and proteomic and bioinformatics analyses were performed to identify differentially expressed proteins and associated pathways. In vitro, a high-glucose inflammatory environment that mimics DCWs was induced in human lymphatic endothelial cells (HLEC). HYSJ and caspase inhibitors were used for intervention. Diverse assays were conducted to assess HLEC function and activation of inflammatory cell death.
Results: The primary constituents of HYSJ unguent included coclaurine, sinapine, and ononin, among others. HYSJ increased healing of DCWs in diabetic mice, protected lymphatic endothelial cells, restored lymphatic drainage in the wound, and upregulated expression of key lymphatic proteins. Numerous proteins, including TLR2, Myd88, STAT1, and inflammatory response-related proteins such as NLRP3, Caspase-1, GSDMD, were expressed differentially in mice. HYSJ unguent also stimulated expression of key lymphatic proteins in HLEC, protected cell function, and suppressed inflammatory cell death.
Conclusion: HYSJ unguent enhances lymphangiogenesis, protects lymphatic endothelial cells in a high-glucose inflammatory environment, and accelerates DCW healing by suppression of TLR2/Myd88/caspase-1 signaling pathway. These findings provide important experimental support for the pharmacological mechanism by which HYSJ unguent facilitates healing of DCWs.
Keywords: traditional Chinese medicine, inflammatory response, TLR2/Myd88 signaling, lymphatic endothelial cells, diabetic wound
Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Xuying Xu, Beijing Hospital of Traditional Chinese Medicine, No. 23, Museum Back Street, Dongcheng District, Beijing, 10010, People’s Republic of China, Tel +86- 010-87906642, Email xxying7341@126.com
Purpose: To investigate the mechanism by which HYSJ unguent promotes lymphangiogenesis and improves the healing of diabetic chronic wounds (DCWs).
Methods: The main components of HYSJ were identified by mass spectrometry. A mouse model with chronic skin ulcers was established. The ultrastructure and lymphatic drainage of lymphatic endothelial cells in wounds were examined. Lymphatic markers in wound tissues were detected, and proteomic and bioinformatics analyses were performed to identify differentially expressed proteins and associated pathways. In vitro, a high-glucose inflammatory environment that mimics DCWs was induced in human lymphatic endothelial cells (HLEC). HYSJ and caspase inhibitors were used for intervention. Diverse assays were conducted to assess HLEC function and activation of inflammatory cell death.
Results: The primary constituents of HYSJ unguent included coclaurine, sinapine, and ononin, among others. HYSJ increased healing of DCWs in diabetic mice, protected lymphatic endothelial cells, restored lymphatic drainage in the wound, and upregulated expression of key lymphatic proteins. Numerous proteins, including TLR2, Myd88, STAT1, and inflammatory response-related proteins such as NLRP3, Caspase-1, GSDMD, were expressed differentially in mice. HYSJ unguent also stimulated expression of key lymphatic proteins in HLEC, protected cell function, and suppressed inflammatory cell death.
Conclusion: HYSJ unguent enhances lymphangiogenesis, protects lymphatic endothelial cells in a high-glucose inflammatory environment, and accelerates DCW healing by suppression of TLR2/Myd88/caspase-1 signaling pathway. These findings provide important experimental support for the pharmacological mechanism by which HYSJ unguent facilitates healing of DCWs.
Keywords: traditional Chinese medicine, inflammatory response, TLR2/Myd88 signaling, lymphatic endothelial cells, diabetic wound