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已发表论文

透明、生物相容性纳米结构表面用于癌细胞捕获与培养

 

Authors Cheng BR, He ZB, Zhao LB, Fang Y, Chen YY, He RX, Chen FF, Song HB, Deng YL, Zhao XZ, Xiong B

Published Date May 2014 Volume 2014:9(1) Pages 2569—2580

DOI http://dx.doi.org/10.2147/IJN.S61233

Received 23 January 2014, Accepted 29 March 2014, Published 22 May 2014

Abstract: Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a “liquid biopsy” and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and efficient CTC detection material made of hydroxyapatite/chitosan (HA/CTS), which is beneficial because of its transparency and excellent biological compatibility. Atomic force microscopy images show that the roughness of the HA/CTS nanofilm (HA/CTSNF) substrates can be controlled by changing the HA:CTS ratio. Enhanced local topographic interactions between nano-components on cancer cell membranes, and the antibody coated nanostructured substrate lead to improved CTC capture and separation. This remarkable nanostructured substrate has the potential for CTC culture in situ and merits further analysis. CTCs captured from artificial blood samples were observed in culture on HA/CTSNF substrates over a period of 14 days by using conventional staining methods (hematoxylin eosin and Wright’s stain). We conclude that these substrates are multifunctional materials capable of isolating and culturing CTCs for subsequent studies.
Keywords: cell capture, cell culture, nanofilms, hydroxyapatite/chitosan






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