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纳米硒化钴过氧化物酶活性及其在尿酸检测中的应用
Authors Zhuang QQ, Lin ZH, Jiang YC, Deng HH, He SB, Su LT, Shi XQ, Chen W
Received 23 November 2016
Accepted for publication 16 March 2017
Published 20 April 2017 Volume 2017:12 Pages 3295—3302
DOI https://doi.org/10.2147/IJN.S128556
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Farooq Shiekh
Peer reviewer comments 5
Editor who approved publication: Dr Linlin Sun
摘要：以钴粉和硒粉为前驱体原料，在无水乙二胺中采用溶剂热法制备了树枝状硒化钴纳米结构。所制备的纳米硒化钴具有过氧化物酶活性，可以催化过氧化氢氧化过氧化物酶底物的反应。基于纳米晶硒化钴的模拟过氧化物酶活性，以 N -乙基-N -(3-磺丙基)-3-甲基苯胺钠盐和 4-氨基安替比林为底物，建立了尿酸的测定方法。在最佳条件下，尿酸测定的线性范围为 2-40 μM，检测限为 0.5 μM。该方法可用于测定人血清样品中的尿酸。
Keywords: enzyme mimics, cobalt selenide,
peroxidase-like activity, uric acid, human serum
