Abstract: A facile method was developed to endow cotton fabric with remarkable antibacterial and ultraviolet (UV)-protective properties. The flower-like TiO₂ micro-nanoparticles were first deposited onto cotton fabric surface via hydrothermal deposition method. Then, the Ag NPs with a high deposition density were evenly formed onto TiO₂@cotton surface by sodium hydroxide solution pretreatment and followed by in situ reduction of ANO₃. This work focused on the influence of different hydrothermal reaction durations and the concentration of AgNO₃ on antibacterial activity against relevant microorganisms in medicine as well as on the UV-blocking property. Ag NPs-loaded TiO₂@cotton exhibited high antibacterial activity with an inhibition rate higher than 99% against Staphylococcus aureus  and Escherichia coli  bacteria. Moreover, the as-prepared cotton fabric coated with Ag NPs and TiO₂ NPs demonstrated outstanding UV protective ability with a high ultraviolet protection factor value of 56.39. Morphological image of the cells revealed a likely loss of viability as a result of the synergistically biocidal effects of TiO₂ and Ag on attached bacteria. These results demonstrate a facile and robust synthesis technology for fabricating multifunctional textiles with a promising biocidal activity against common Gram-negative and Gram-positive bacteria.
Keywords: TiO₂ nanoparticles, Ag nanoparticles, fabric, antibacterial, UV-shielding, biomedical