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Authors Su D, Liu Y, Song T
Received 23 November 2016
Accepted for publication 23 January 2017
Published 13 March 2017 Volume 2017:10 Pages 1549—1559
DOI https://doi.org/10.2147/OTT.S128564
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Ashok Kumar Pandurangan
Peer reviewer comments 2
Editor who approved publication: Dr Jianmin Xu
Background: Thyroid cancer is the most common endocrine malignant disease
with a high incidence rate. The expression of IQGAP1 is upregulated in various
cancers, including thyroid cancer. However, the role and underlying mechanism
of IQGAP1 in thyroid cancer are still not clear.
Materials and methods: The expression of IQGAP1 in thyroid cancer tissues and
cells was determined by reverse transcription polymerase chain reaction and
Western blot analysis. Cells were transfected with different siRNAs using
Lipofectamine 2000 or were treated with various concentrations of XAV939. The
effects of IQGAP1 knockdown on proliferation and epithelial–mesenchymal
transition (EMT) of thyroid cancer cells were determined by MTT assay and
Western blot analysis. Animal experiments were performed to investigate the
effects of IQGAP1 knockdown on the growth of tumors in vivo.
Results: High IQGAP1 expression is found in thyroid cancer
tissues and cells. Knockdown of IQGAP1 had inhibitory effects on cell
proliferation and EMT, as well as on the Wnt/β-catenin pathway. Additionally,
inactivation of the Wnt/β-catenin pathway by XAV939 or si-β-catenin suppressed
cell proliferation and EMT. Furthermore, suppression of the Wnt/β-catenin
pathway reversed the positive effects of pcDNA-IQGAP1 on cell proliferation and
EMT in vitro. Moreover, downregulation of IQGAP1 suppressed tumor growth and
EMT in SW579 tumor xenografts through the Wnt/β-catenin pathway in vivo.
Conclusion: Our study demonstrated that knockdown
of IQGAP1 inhibited cell proliferation and EMT through blocking the
Wnt/β-catenin pathway in thyroid cancer.
Keywords: IQGAP1, thyroid cancer, proliferation,
epithelial–mesenchymal transition, Wnt/β-catenin