已发表论文

— 血清应答因子核易位的降低与香烟烟雾诱导的 COPD 模型小鼠的骨骼肌萎缩相关

 

Authors Ma R, Gong XF, Jiang H, Lin CY, Chen YQ, Xu XM, Zhang CT, Wang J, Lu WJ, Zhong NS

Received 25 March 2016

Accepted for publication 5 September 2016

Published 20 February 2017 Volume 2017:12 Pages 581—587

DOI https://doi.org/10.2147/COPD.S109243

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 3

Editor who approved publication: Dr Richard Russell

Abstract: Skeletal muscle atrophy and dysfunction are common complications in the chronic obstructive pulmonary disease (COPD). However, the underlying molecular mechanism remains elusive. Serum response factor (SRF) is a transcription factor which is critical in myocyte differentiation and growth. In this study, we established a mouse COPD model induced by cigarette smoking (CS) exposure for 24 weeks, with apparent pathophysiological changes, including increased airway resistance, enlarged alveoli, and skeletal muscle atrophy. Levels of upstream regulators of SRF, striated muscle activator of Rho signaling (STARS), and ras homolog gene family, member A (RhoA) were decreased in quadriceps muscle of COPD mice. Meanwhile, the nucleic location of SRF was diminished along with its cytoplasmic accumulation. There was a downregulation of the target muscle-specific gene, Igf1 . These results suggest that the CS is one of the major cause for COPD pathogenesis, which induces the COPD-associated skeletal muscle atrophy which is closely related to decreasing SRF nucleic translocation, consequently downregulating the SRF target genes involved in muscle growth and nutrition. The STARS/RhoA signaling pathway might contribute to this course by impacting SRF subcellular distribution.
Keywords: SRF, chronic obstructive pulmonary disease, skeletal muscle atrophy, cigarette smoking


* 在 583 页,在左栏,最后一段,句子 “…the primer for the Igf1 gene forward: 5′-tgctgtccctctatgcttcc-3′, reverse: 5′-gaaggaatagccacgctcag-3′” 应该是:" … forward: 5′-tggatgctcttcagttcgtg-3′, reverse: 5′-cacaatgcctgtctgaggtg-3′” 。

 

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