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Authors Zhuang PH, Xu L, Gao L, Lu W, Ruan LT, Yang J
Received 22 May 2016
Accepted for publication 17 September 2016
Published 14 February 2017 Volume 2017:10 Pages 847—857
DOI https://doi.org/10.2147/OTT.S113353
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Akshita Wason
Peer reviewer comments 3
Editor who approved publication: Dr Samir Farghaly
***本文章已被撤回***
Abstract: The study is designed
to explore the correlations of microvascular blood flow of contrast-enhanced
ultrasound (CEUS) and hepatocyte growth factor (HGF)/c-Met signaling pathway
with clinicopathological features and prognosis of patients with hepatocellular
carcinoma (HCC). One hundred and eighteen patients pathologically diagnosed as
primary HCC were selected. All HCC patients underwent CEUS examination before
operation. HCC tissues and adjacent normal tissue specimens were obtained to
detect the protein rates of HGF and c-Met expressions by immunohistochemistry.
The mRNA expressions of HGF and c-Met were detected by quantitative real-time
polymerase chase reaction assay. The microvessel density (MVD) was tested by
CD34 immunohistochemistry. Compared with liver parenchyma, the HCC lesions had
higher MVD, preoperative peak intensity (PI), area under the curve (AUC), lower
preoperative time to peak (TTP), and washout time (WOT). Compared with adjacent
normal tissues, the protein and mRNA expressions of HGF were reduced in HCC
tissues, but the protein and mRNA expressions of c-Met and MVD were increased.
The protein expressions of HGF and c-Met exhibited evident correlations with
TNM stage, tumor size, vascular invasion, liver cirrhosis, and hepatitis B
virus and hepatitis C virus infection of HCC patients. The tumor size and
number, vascular invasion, the protein expressions of HGF and c-Met, and MVD
were associated with the TTP, PI, WOT, and AUC of CEUS in HCC patients. The
protein expressions of HGF and c-Met, MVD and preoperative PI revealed negative
associations with the prognosis of HCC patients. In conclusion, quantitative
parameters of CEUS and HGF/c-Met signaling pathway-related proteins may be
helpful for early diagnosis and prognosis prediction of HCC patients.
Keywords: hepatocellular carcinoma, hepatocyte
growth factor, cellular-mesenchymal to epithelial transition factor, signaling
pathway, microvascular blood flow, contrast-enhanced ultrasound,
clinicopathological feature