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静电纺丝制备明胶/PCL 和胶原蛋白/ PLCL 支架的血管组织工程

 

Authors Fu W, Liu Z, Feng B, Hu R, He X, Wang H, Yin M, Huang H, Zhang H, Wang W

Published Date May 2014 Volume 2014:9(1) Pages 2335—2344

DOI http://dx.doi.org/10.2147/IJN.S61375

Received 26 January 2014, Accepted 10 March 2014, Published 13 May 2014

 
Abstract: Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun gelatin/polycaprolactone (PCL) and collagen/poly(l-lactic acid-co-ε-caprolactone) (PLCL) scaffolds were successfully produced. Scanning electron micrographs showed that fibers of both membranes were smooth and homogeneous. Water contact angle measurements further demonstrated that both scaffolds were hydrophilic. To determine cell attachment and migration on the scaffolds, both hybrid scaffolds were seeded with human umbilical arterial smooth muscle cells. Scanning electron micrographs and MTT assays showed that the cells grew and proliferated well on both hybrid scaffolds. Gross observation of the transplanted scaffolds revealed that the engineered collagen/PLCL scaffolds were smoother and brighter than the gelatin/PCL scaffolds. Hematoxylin and eosin staining showed that the engineered blood vessels constructed by collagen/PLCL electrospun membranes formed relatively homogenous vessel-like tissues. Interestingly, Young's modulus for the engineered collagen/PLCL scaffolds was greater than for the gelatin/PCL scaffolds. Together, these results indicate that nanofibrous collagen/PLCL membranes with favorable mechanical and biological properties may be a desirable scaffold for vascular tissue engineering.
Keywords: electrospinning, gelatin, collagen, polycaprolactone, poly(l-lactic acid-co-ε-caprolactone)







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